Cytoplasmic localization of Wis1 MAPKK by nuclear export signal is important for nuclear targeting of Spc1/Sty1 MAPK in fission yeast

Aaron Ngocky Nguyen, Aminah D. Ikner, Mitsue Shiozaki, Sasha M. Warren, Kazuhiro Shiozaki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Mitogen-activated protein kinase (MAPK) cascade is a ubiquitous signaling module that transmits extracellular stimuli through the cytoplasm to the nucleus; in response to activating stimuli, MAPKs translocate into the nucleus. Mammalian MEK MAPK kinases (MAPKKs) have in their N termini an MAPK-docking site and a nuclear export signal (NES) sequence, which are known to play critical roles in maintaining ERK MAPKs in the cytoplasm of unstimulated cells. Herein, we show that the Wis1 MAPKK of the stress-activated Spc1 MAPK cascade in fission yeast also has a MAPK-docking site and an NES sequence in its N-terminal domain. Unexpectedly, an inactivating mutation to the NES of chromosomal wis1+ does not affect the subcellular localization of Spc1 MAPK, whereas this NES mutation disturbs the cytoplasmic localization of Wis1. However, when Wis1 is targeted to the nucleus by fusing to a nuclear localization signal sequence, stress-induced nuclear translocation of Spc1 is abrogated, indicating that cytoplasmic Wis1 is required for nuclear transport of Spc1 upon stress. Moreover, we have observed that a fraction of Wis1 translocates into the nucleus in response to stress. These results suggest that cytoplasmic localization of Wis1 MAPKK by its NES is important for stress signaling to the nucleus.

Original languageEnglish (US)
Pages (from-to)2651-2663
Number of pages13
JournalMolecular Biology of the Cell
Volume13
Issue number8
DOIs
StatePublished - Aug 2002

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Nuclear Export Signals
Schizosaccharomyces
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Protein Sorting Signals
Cytoplasm
Nuclear Localization Signals
Mutation
Cell Nucleus Active Transport

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cytoplasmic localization of Wis1 MAPKK by nuclear export signal is important for nuclear targeting of Spc1/Sty1 MAPK in fission yeast. / Nguyen, Aaron Ngocky; Ikner, Aminah D.; Shiozaki, Mitsue; Warren, Sasha M.; Shiozaki, Kazuhiro.

In: Molecular Biology of the Cell, Vol. 13, No. 8, 08.2002, p. 2651-2663.

Research output: Contribution to journalArticle

Nguyen, Aaron Ngocky ; Ikner, Aminah D. ; Shiozaki, Mitsue ; Warren, Sasha M. ; Shiozaki, Kazuhiro. / Cytoplasmic localization of Wis1 MAPKK by nuclear export signal is important for nuclear targeting of Spc1/Sty1 MAPK in fission yeast. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 8. pp. 2651-2663.
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