Cytoplasmic ATP inhibition of CLC-1 is enhanced by low pH

Pang Yen Tseng, Brett Bennetts, Tsung-Yu Chen

Research output: Contribution to journalArticle

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Abstract

The CLC-1 Cl- channel is abundantly expressed on the plasma membrane of muscle cells, and the membrane potential of muscle cells is largely controlled by the activity of this Cl- channel. Previous studies showed that low intracellular pH increases the overall open probability of recombinant CLC-1 channels in various expression systems. Low intracellular pH, however, is known to inhibit the Cl- conductance on the native muscle membrane, contradicting the findings from the recombinant CLC-1 channels in expressed systems. Here we show that in the presence of physiological concentrations of ATP, reduction of the intracellular pH indeed inhibits the expressed CLC-1, mostly by decreasing the open probability of the common gate of the channel.

Original languageEnglish (US)
Pages (from-to)217-221
Number of pages5
JournalJournal of General Physiology
Volume130
Issue number2
DOIs
StatePublished - Aug 2007

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ASJC Scopus subject areas

  • Physiology

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