Abstract
The pathogenesis of encephalitis due to cytomegalovirus (CMV), particularly the role of microglial cells in the spread or control of infection, remains incompletely defined. In this study, microglial cells were isolated from the brains of newborn mice and infected in vitro with murine CMV (MCMV). Microglial cells supported productive MCMV replication, and the MCMV-infected microglia manifested a cytopathic effect (CPE) characteristic of CMV infection. Exposure of microglia to interferon-γ (IFN-γ) 24 h before infection markedly suppressed virus production and resultant CPE in a dose- dependent fashion. Furthermore, the addition of IFN-γ 2 h after infection demonstrated an antiviral effect equivalent to that achieved when IFN-γ was administered 2 h before infection. These results demonstrate that murine microglial cells are fully permissive to MCMV replication and that IFN-γ markedly suppresses virus expression in these cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1092-1096 |
| Number of pages | 5 |
| Journal | Journal of Infectious Diseases |
| Volume | 169 |
| Issue number | 5 |
| State | Published - May 1994 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Public Health, Environmental and Occupational Health
Cite this
Cytomegalovirus replication in murine microglial cell cultures : Suppression of permissive infection by interferon-γ. / Schut, Ronald L.; Gekker, Genya; Hu, Su; Chao, Chun C.; Pomeroy, Claire; Colin Jordan, M.; Peterson, Phillip K.
In: Journal of Infectious Diseases, Vol. 169, No. 5, 05.1994, p. 1092-1096.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytomegalovirus replication in murine microglial cell cultures
T2 - Suppression of permissive infection by interferon-γ
AU - Schut, Ronald L.
AU - Gekker, Genya
AU - Hu, Su
AU - Chao, Chun C.
AU - Pomeroy, Claire
AU - Colin Jordan, M.
AU - Peterson, Phillip K.
PY - 1994/5
Y1 - 1994/5
N2 - The pathogenesis of encephalitis due to cytomegalovirus (CMV), particularly the role of microglial cells in the spread or control of infection, remains incompletely defined. In this study, microglial cells were isolated from the brains of newborn mice and infected in vitro with murine CMV (MCMV). Microglial cells supported productive MCMV replication, and the MCMV-infected microglia manifested a cytopathic effect (CPE) characteristic of CMV infection. Exposure of microglia to interferon-γ (IFN-γ) 24 h before infection markedly suppressed virus production and resultant CPE in a dose- dependent fashion. Furthermore, the addition of IFN-γ 2 h after infection demonstrated an antiviral effect equivalent to that achieved when IFN-γ was administered 2 h before infection. These results demonstrate that murine microglial cells are fully permissive to MCMV replication and that IFN-γ markedly suppresses virus expression in these cells.
AB - The pathogenesis of encephalitis due to cytomegalovirus (CMV), particularly the role of microglial cells in the spread or control of infection, remains incompletely defined. In this study, microglial cells were isolated from the brains of newborn mice and infected in vitro with murine CMV (MCMV). Microglial cells supported productive MCMV replication, and the MCMV-infected microglia manifested a cytopathic effect (CPE) characteristic of CMV infection. Exposure of microglia to interferon-γ (IFN-γ) 24 h before infection markedly suppressed virus production and resultant CPE in a dose- dependent fashion. Furthermore, the addition of IFN-γ 2 h after infection demonstrated an antiviral effect equivalent to that achieved when IFN-γ was administered 2 h before infection. These results demonstrate that murine microglial cells are fully permissive to MCMV replication and that IFN-γ markedly suppresses virus expression in these cells.
UR - http://www.scopus.com/inward/record.url?scp=0028038078&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028038078&partnerID=8YFLogxK
M3 - Article
C2 - 8169398
AN - SCOPUS:0028038078
VL - 169
SP - 1092
EP - 1096
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 5
ER -