Cytomegalovirus replication in murine microglial cell cultures: Suppression of permissive infection by interferon-γ

Ronald L. Schut, Genya Gekker, Su Hu, Chun C. Chao, Claire Pomeroy, M. Colin Jordan, Phillip K. Peterson

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The pathogenesis of encephalitis due to cytomegalovirus (CMV), particularly the role of microglial cells in the spread or control of infection, remains incompletely defined. In this study, microglial cells were isolated from the brains of newborn mice and infected in vitro with murine CMV (MCMV). Microglial cells supported productive MCMV replication, and the MCMV-infected microglia manifested a cytopathic effect (CPE) characteristic of CMV infection. Exposure of microglia to interferon-γ (IFN-γ) 24 h before infection markedly suppressed virus production and resultant CPE in a dose- dependent fashion. Furthermore, the addition of IFN-γ 2 h after infection demonstrated an antiviral effect equivalent to that achieved when IFN-γ was administered 2 h before infection. These results demonstrate that murine microglial cells are fully permissive to MCMV replication and that IFN-γ markedly suppresses virus expression in these cells.

Original languageEnglish (US)
Pages (from-to)1092-1096
Number of pages5
JournalJournal of Infectious Diseases
Volume169
Issue number5
StatePublished - May 1994
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

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    Schut, R. L., Gekker, G., Hu, S., Chao, C. C., Pomeroy, C., Colin Jordan, M., & Peterson, P. K. (1994). Cytomegalovirus replication in murine microglial cell cultures: Suppression of permissive infection by interferon-γ. Journal of Infectious Diseases, 169(5), 1092-1096.