Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods

Leonard Eisenfeld, James C. McLaughlin, Donald Mayo, Paula Klevjer-Anderson, Herbert Silver, Peter Krause, Janice Anderson, Victor Herson, John Savidakis, Ann Marie Lazar, Ted Rosenkrantz, Hema DeSilva, Ray Ryan, Debra A. LeConche, Terese Donovan, Kathleen Ellis, Donna Buchanan, John Raye, Patricia Pisciotto, Dempsey RoweIsabella Knox, Anthony F Philipps, Debra Whiteley, Jean Apollonio, James Schick, Michelle Conlon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (<1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mothers of premature infants. Plasma from blood donors and sera from mothers were tested by either latex agglutination (LA) or by an immunofluorescent antibody assay (IFA), and results were compared with those from an enzymelinked immunosorbent assay (ELISA). Sensitivity and specificity for LA to ELISA were significantly better than for IFA to ELISA [sensitivity 79/81 (97%) vs 171/202 (85%), and specificity 90/94 (96%) vs 257/347 (74%), p < 0.01]. These differences remained whether plasma or sera were tested. Borderline values explained only two (33%) of six LA-ELISA as well as only 70 (58%) of 121 IFA-ELISA discordance. CMV seroprevalence rate for the donor blood population was 38%, and for the mothers was 53%. The LA assay is superior to the IFA assay for CMV screening of blood donors and maternal populations.

Original languageEnglish (US)
Pages (from-to)125-128
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume15
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Very Low Birth Weight Infant
Immunosorbents
Blood Donors
Cytomegalovirus
Agglutination
Latex
Mothers
Newborn Infant
Antibodies
Seroepidemiologic Studies
Serology
Serum
Premature Infants
Population
Sensitivity and Specificity

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods. / Eisenfeld, Leonard; McLaughlin, James C.; Mayo, Donald; Klevjer-Anderson, Paula; Silver, Herbert; Krause, Peter; Anderson, Janice; Herson, Victor; Savidakis, John; Lazar, Ann Marie; Rosenkrantz, Ted; DeSilva, Hema; Ryan, Ray; LeConche, Debra A.; Donovan, Terese; Ellis, Kathleen; Buchanan, Donna; Raye, John; Pisciotto, Patricia; Rowe, Dempsey; Knox, Isabella; Philipps, Anthony F; Whiteley, Debra; Apollonio, Jean; Schick, James; Conlon, Michelle.

In: Diagnostic Microbiology and Infectious Disease, Vol. 15, No. 2, 1992, p. 125-128.

Research output: Contribution to journalArticle

Eisenfeld, L, McLaughlin, JC, Mayo, D, Klevjer-Anderson, P, Silver, H, Krause, P, Anderson, J, Herson, V, Savidakis, J, Lazar, AM, Rosenkrantz, T, DeSilva, H, Ryan, R, LeConche, DA, Donovan, T, Ellis, K, Buchanan, D, Raye, J, Pisciotto, P, Rowe, D, Knox, I, Philipps, AF, Whiteley, D, Apollonio, J, Schick, J & Conlon, M 1992, 'Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods', Diagnostic Microbiology and Infectious Disease, vol. 15, no. 2, pp. 125-128. https://doi.org/10.1016/0732-8893(92)90035-R
Eisenfeld, Leonard ; McLaughlin, James C. ; Mayo, Donald ; Klevjer-Anderson, Paula ; Silver, Herbert ; Krause, Peter ; Anderson, Janice ; Herson, Victor ; Savidakis, John ; Lazar, Ann Marie ; Rosenkrantz, Ted ; DeSilva, Hema ; Ryan, Ray ; LeConche, Debra A. ; Donovan, Terese ; Ellis, Kathleen ; Buchanan, Donna ; Raye, John ; Pisciotto, Patricia ; Rowe, Dempsey ; Knox, Isabella ; Philipps, Anthony F ; Whiteley, Debra ; Apollonio, Jean ; Schick, James ; Conlon, Michelle. / Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods. In: Diagnostic Microbiology and Infectious Disease. 1992 ; Vol. 15, No. 2. pp. 125-128.
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abstract = "We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (<1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mothers of premature infants. Plasma from blood donors and sera from mothers were tested by either latex agglutination (LA) or by an immunofluorescent antibody assay (IFA), and results were compared with those from an enzymelinked immunosorbent assay (ELISA). Sensitivity and specificity for LA to ELISA were significantly better than for IFA to ELISA [sensitivity 79/81 (97{\%}) vs 171/202 (85{\%}), and specificity 90/94 (96{\%}) vs 257/347 (74{\%}), p < 0.01]. These differences remained whether plasma or sera were tested. Borderline values explained only two (33{\%}) of six LA-ELISA as well as only 70 (58{\%}) of 121 IFA-ELISA discordance. CMV seroprevalence rate for the donor blood population was 38{\%}, and for the mothers was 53{\%}. The LA assay is superior to the IFA assay for CMV screening of blood donors and maternal populations.",
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AU - Eisenfeld, Leonard

AU - McLaughlin, James C.

AU - Mayo, Donald

AU - Klevjer-Anderson, Paula

AU - Silver, Herbert

AU - Krause, Peter

AU - Anderson, Janice

AU - Herson, Victor

AU - Savidakis, John

AU - Lazar, Ann Marie

AU - Rosenkrantz, Ted

AU - DeSilva, Hema

AU - Ryan, Ray

AU - LeConche, Debra A.

AU - Donovan, Terese

AU - Ellis, Kathleen

AU - Buchanan, Donna

AU - Raye, John

AU - Pisciotto, Patricia

AU - Rowe, Dempsey

AU - Knox, Isabella

AU - Philipps, Anthony F

AU - Whiteley, Debra

AU - Apollonio, Jean

AU - Schick, James

AU - Conlon, Michelle

PY - 1992

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N2 - We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (<1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mothers of premature infants. Plasma from blood donors and sera from mothers were tested by either latex agglutination (LA) or by an immunofluorescent antibody assay (IFA), and results were compared with those from an enzymelinked immunosorbent assay (ELISA). Sensitivity and specificity for LA to ELISA were significantly better than for IFA to ELISA [sensitivity 79/81 (97%) vs 171/202 (85%), and specificity 90/94 (96%) vs 257/347 (74%), p < 0.01]. These differences remained whether plasma or sera were tested. Borderline values explained only two (33%) of six LA-ELISA as well as only 70 (58%) of 121 IFA-ELISA discordance. CMV seroprevalence rate for the donor blood population was 38%, and for the mothers was 53%. The LA assay is superior to the IFA assay for CMV screening of blood donors and maternal populations.

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