Abstract
We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (<1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mothers of premature infants. Plasma from blood donors and sera from mothers were tested by either latex agglutination (LA) or by an immunofluorescent antibody assay (IFA), and results were compared with those from an enzymelinked immunosorbent assay (ELISA). Sensitivity and specificity for LA to ELISA were significantly better than for IFA to ELISA [sensitivity 79/81 (97%) vs 171/202 (85%), and specificity 90/94 (96%) vs 257/347 (74%), p < 0.01]. These differences remained whether plasma or sera were tested. Borderline values explained only two (33%) of six LA-ELISA as well as only 70 (58%) of 121 IFA-ELISA discordance. CMV seroprevalence rate for the donor blood population was 38%, and for the mothers was 53%. The LA assay is superior to the IFA assay for CMV screening of blood donors and maternal populations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-128 |
| Number of pages | 4 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Volume | 15 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
- Microbiology
- Parasitology
- Virology
- Immunology and Allergy
- Infectious Diseases
Cite this
Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods. / Eisenfeld, Leonard; McLaughlin, James C.; Mayo, Donald; Klevjer-Anderson, Paula; Silver, Herbert; Krause, Peter; Anderson, Janice; Herson, Victor; Savidakis, John; Lazar, Ann Marie; Rosenkrantz, Ted; DeSilva, Hema; Ryan, Ray; LeConche, Debra A.; Donovan, Terese; Ellis, Kathleen; Buchanan, Donna; Raye, John; Pisciotto, Patricia; Rowe, Dempsey; Knox, Isabella; Philipps, Anthony F; Whiteley, Debra; Apollonio, Jean; Schick, James; Conlon, Michelle.
In: Diagnostic Microbiology and Infectious Disease, Vol. 15, No. 2, 1992, p. 125-128.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods
AU - Eisenfeld, Leonard
AU - McLaughlin, James C.
AU - Mayo, Donald
AU - Klevjer-Anderson, Paula
AU - Silver, Herbert
AU - Krause, Peter
AU - Anderson, Janice
AU - Herson, Victor
AU - Savidakis, John
AU - Lazar, Ann Marie
AU - Rosenkrantz, Ted
AU - DeSilva, Hema
AU - Ryan, Ray
AU - LeConche, Debra A.
AU - Donovan, Terese
AU - Ellis, Kathleen
AU - Buchanan, Donna
AU - Raye, John
AU - Pisciotto, Patricia
AU - Rowe, Dempsey
AU - Knox, Isabella
AU - Philipps, Anthony F
AU - Whiteley, Debra
AU - Apollonio, Jean
AU - Schick, James
AU - Conlon, Michelle
PY - 1992
Y1 - 1992
N2 - We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (<1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mothers of premature infants. Plasma from blood donors and sera from mothers were tested by either latex agglutination (LA) or by an immunofluorescent antibody assay (IFA), and results were compared with those from an enzymelinked immunosorbent assay (ELISA). Sensitivity and specificity for LA to ELISA were significantly better than for IFA to ELISA [sensitivity 79/81 (97%) vs 171/202 (85%), and specificity 90/94 (96%) vs 257/347 (74%), p < 0.01]. These differences remained whether plasma or sera were tested. Borderline values explained only two (33%) of six LA-ELISA as well as only 70 (58%) of 121 IFA-ELISA discordance. CMV seroprevalence rate for the donor blood population was 38%, and for the mothers was 53%. The LA assay is superior to the IFA assay for CMV screening of blood donors and maternal populations.
AB - We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (<1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mothers of premature infants. Plasma from blood donors and sera from mothers were tested by either latex agglutination (LA) or by an immunofluorescent antibody assay (IFA), and results were compared with those from an enzymelinked immunosorbent assay (ELISA). Sensitivity and specificity for LA to ELISA were significantly better than for IFA to ELISA [sensitivity 79/81 (97%) vs 171/202 (85%), and specificity 90/94 (96%) vs 257/347 (74%), p < 0.01]. These differences remained whether plasma or sera were tested. Borderline values explained only two (33%) of six LA-ELISA as well as only 70 (58%) of 121 IFA-ELISA discordance. CMV seroprevalence rate for the donor blood population was 38%, and for the mothers was 53%. The LA assay is superior to the IFA assay for CMV screening of blood donors and maternal populations.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026601860&partnerID=8YFLogxK
U2 - 10.1016/0732-8893(92)90035-R
DO - 10.1016/0732-8893(92)90035-R
M3 - Article
C2 - 1315230
AN - SCOPUS:0026601860
VL - 15
SP - 125
EP - 128
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 2
ER -