Cytomegalovirus activates transcription directed by the long terminal repeat of human immunodeficiency virus type 1

Peter A Barry, Elissa Pratt-Lowe, B. Matija Peterlin, Paul A Luciw

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Proteins encoded by a variety of DNA viruses activate gene expression from the promoter within the long terminal repeat (LTR) of the human immunodeficiency virus type 1 (HIV-1). The mechanism by which immediate-early (IE) gene products of human cytomegalovirus (CMV) activate expression from the HIV-1 LTR was examined in transient expression assays in cultures of human cells by using plasmids containing the LTR linked to the bacterial chloramphenicol acetyltransferase (CAT) gene and a plasmid expressing the CMV IE gene. Analysis of clustered site mutations within the HIV-1 LTR revealed that sequences from nucleotides -6 to +20 (relative to the start site of transcription) are critical for responsiveness to transactivation by CMV IE gene products. This region partially overlaps the trans-acting response element (+19 to +42) required for function of the HIV-1 transactivator. The CMV IE gene was shown to increase the steady-state levels of both prematurely terminated and full-length transcripts initiated within the LTR. These results support a model in which CMV IE gene products act through a specific regulatory element in the HIV-1 LTR to increase viral transcription.

Original languageEnglish (US)
Pages (from-to)2932-2940
Number of pages9
JournalJournal of Virology
Volume64
Issue number6
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology

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