Cytological studies of recombination in rhesus males

T. Hassold, T. Hansen, P. Hunt, C. Vandevoort

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

An immunofluorescence approach was used to directly examine meiotic recombination events in 483 pachytene spermatocytes from 11 male rhesus monkeys. Specifically, we examined the nuclear localization patterns of the DNA mismatch repair protein MLH1, known from analyses of other mammalian species to be a useful marker of meiotic cross-overs. Our results indicated that rhesus pachytene spermatocytes contain approximately 40 cross-overs per cell, corresponding to about one cross-over per chromosome. The chromosomal distribution of these exchanges was consistent with data from human and mouse males but, surprisingly, the overall number of foci was lower, and the number of 'exchangeless' bivalents higher, than reported for either humans or mice.

Original languageEnglish (US)
Pages (from-to)132-138
Number of pages7
JournalCytogenetic and Genome Research
Volume124
Issue number2
DOIs
StatePublished - May 2009

Fingerprint

Spermatocytes
Genetic Recombination
DNA Mismatch Repair
Macaca mulatta
Fluorescent Antibody Technique
Chromosomes
MutL Protein Homolog 1

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Hassold, T., Hansen, T., Hunt, P., & Vandevoort, C. (2009). Cytological studies of recombination in rhesus males. Cytogenetic and Genome Research, 124(2), 132-138. https://doi.org/10.1159/000207519

Cytological studies of recombination in rhesus males. / Hassold, T.; Hansen, T.; Hunt, P.; Vandevoort, C.

In: Cytogenetic and Genome Research, Vol. 124, No. 2, 05.2009, p. 132-138.

Research output: Contribution to journalArticle

Hassold, T, Hansen, T, Hunt, P & Vandevoort, C 2009, 'Cytological studies of recombination in rhesus males', Cytogenetic and Genome Research, vol. 124, no. 2, pp. 132-138. https://doi.org/10.1159/000207519
Hassold, T. ; Hansen, T. ; Hunt, P. ; Vandevoort, C. / Cytological studies of recombination in rhesus males. In: Cytogenetic and Genome Research. 2009 ; Vol. 124, No. 2. pp. 132-138.
@article{0b4a12a9783d41e791ad713b2a68d530,
title = "Cytological studies of recombination in rhesus males",
abstract = "An immunofluorescence approach was used to directly examine meiotic recombination events in 483 pachytene spermatocytes from 11 male rhesus monkeys. Specifically, we examined the nuclear localization patterns of the DNA mismatch repair protein MLH1, known from analyses of other mammalian species to be a useful marker of meiotic cross-overs. Our results indicated that rhesus pachytene spermatocytes contain approximately 40 cross-overs per cell, corresponding to about one cross-over per chromosome. The chromosomal distribution of these exchanges was consistent with data from human and mouse males but, surprisingly, the overall number of foci was lower, and the number of 'exchangeless' bivalents higher, than reported for either humans or mice.",
author = "T. Hassold and T. Hansen and P. Hunt and C. Vandevoort",
year = "2009",
month = "5",
doi = "10.1159/000207519",
language = "English (US)",
volume = "124",
pages = "132--138",
journal = "Cytogenetic and Genome Research",
issn = "1424-8581",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Cytological studies of recombination in rhesus males

AU - Hassold, T.

AU - Hansen, T.

AU - Hunt, P.

AU - Vandevoort, C.

PY - 2009/5

Y1 - 2009/5

N2 - An immunofluorescence approach was used to directly examine meiotic recombination events in 483 pachytene spermatocytes from 11 male rhesus monkeys. Specifically, we examined the nuclear localization patterns of the DNA mismatch repair protein MLH1, known from analyses of other mammalian species to be a useful marker of meiotic cross-overs. Our results indicated that rhesus pachytene spermatocytes contain approximately 40 cross-overs per cell, corresponding to about one cross-over per chromosome. The chromosomal distribution of these exchanges was consistent with data from human and mouse males but, surprisingly, the overall number of foci was lower, and the number of 'exchangeless' bivalents higher, than reported for either humans or mice.

AB - An immunofluorescence approach was used to directly examine meiotic recombination events in 483 pachytene spermatocytes from 11 male rhesus monkeys. Specifically, we examined the nuclear localization patterns of the DNA mismatch repair protein MLH1, known from analyses of other mammalian species to be a useful marker of meiotic cross-overs. Our results indicated that rhesus pachytene spermatocytes contain approximately 40 cross-overs per cell, corresponding to about one cross-over per chromosome. The chromosomal distribution of these exchanges was consistent with data from human and mouse males but, surprisingly, the overall number of foci was lower, and the number of 'exchangeless' bivalents higher, than reported for either humans or mice.

UR - http://www.scopus.com/inward/record.url?scp=65649140050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65649140050&partnerID=8YFLogxK

U2 - 10.1159/000207519

DO - 10.1159/000207519

M3 - Article

VL - 124

SP - 132

EP - 138

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 2

ER -