Abstract
Immunofluorescence and electron microscopy were used to study the development and cytological distribution of dengue 2 virion and nonvirion antigens in monkey kidney cells. The type and combination of fixatives were found to affect the intensity of fluorescence. Paraformaldehyde fixation alone resulted in a low level of fluorescence, but an additional fixation step with ethanol or acetone resulted in maximum staining. No fluorescence was obtained after fixation with glutaraldehyde alone or in combination with other solvents. Methanol caused selective ablation of fluorescence by antibody to purified virions. Dengue specific fluorescence that was most intense in the perinuclear area radiated in a granular pattern of decreasing intensity into the cytoplasm. The perinuclear fluorescence was associated with nonvirion antigens and the cytoplasmic fluorescence was associated with virion antigens. Electron micrographs of infected cells revealed vesicular bodies with reticular electron dense centers, clusters of structurally complete virions deep within the endoplasmic reticulum, and single virus particles in vacuoles near the periphery of the cell.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 809-816 |
| Number of pages | 8 |
| Journal | Infection and Immunity |
| Volume | 7 |
| Issue number | 5 |
| State | Published - Dec 1 1973 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Cytological localization of dengue 2 antigens : an immunological study with ultrastructural correlation. / Cardiff, Robert; Russ, S. B.; Brandt, W. E.; Russell, P. K.
In: Infection and Immunity, Vol. 7, No. 5, 01.12.1973, p. 809-816.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytological localization of dengue 2 antigens
T2 - an immunological study with ultrastructural correlation
AU - Cardiff, Robert
AU - Russ, S. B.
AU - Brandt, W. E.
AU - Russell, P. K.
PY - 1973/12/1
Y1 - 1973/12/1
N2 - Immunofluorescence and electron microscopy were used to study the development and cytological distribution of dengue 2 virion and nonvirion antigens in monkey kidney cells. The type and combination of fixatives were found to affect the intensity of fluorescence. Paraformaldehyde fixation alone resulted in a low level of fluorescence, but an additional fixation step with ethanol or acetone resulted in maximum staining. No fluorescence was obtained after fixation with glutaraldehyde alone or in combination with other solvents. Methanol caused selective ablation of fluorescence by antibody to purified virions. Dengue specific fluorescence that was most intense in the perinuclear area radiated in a granular pattern of decreasing intensity into the cytoplasm. The perinuclear fluorescence was associated with nonvirion antigens and the cytoplasmic fluorescence was associated with virion antigens. Electron micrographs of infected cells revealed vesicular bodies with reticular electron dense centers, clusters of structurally complete virions deep within the endoplasmic reticulum, and single virus particles in vacuoles near the periphery of the cell.
AB - Immunofluorescence and electron microscopy were used to study the development and cytological distribution of dengue 2 virion and nonvirion antigens in monkey kidney cells. The type and combination of fixatives were found to affect the intensity of fluorescence. Paraformaldehyde fixation alone resulted in a low level of fluorescence, but an additional fixation step with ethanol or acetone resulted in maximum staining. No fluorescence was obtained after fixation with glutaraldehyde alone or in combination with other solvents. Methanol caused selective ablation of fluorescence by antibody to purified virions. Dengue specific fluorescence that was most intense in the perinuclear area radiated in a granular pattern of decreasing intensity into the cytoplasm. The perinuclear fluorescence was associated with nonvirion antigens and the cytoplasmic fluorescence was associated with virion antigens. Electron micrographs of infected cells revealed vesicular bodies with reticular electron dense centers, clusters of structurally complete virions deep within the endoplasmic reticulum, and single virus particles in vacuoles near the periphery of the cell.
UR - http://www.scopus.com/inward/record.url?scp=0015877443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015877443&partnerID=8YFLogxK
M3 - Article
C2 - 4202963
AN - SCOPUS:0015877443
VL - 7
SP - 809
EP - 816
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 5
ER -