Cytologic-histologic concordance in the diagnosis of neoplasia in canine and feline lymph nodes: A retrospective study of 367 cases

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Abstract

Lymph nodes are frequently sampled in dogs and cats for the diagnosis of primary and metastatic neoplasia. We determined the accuracy of cytologic diagnosis in lymph nodes using histology as the gold standard. Lymph node reports (2001-2011) were retrospectively evaluated and diagnoses were categorized as neoplastic or non-neoplastic. Lymph nodes from 296 dogs and 71 cats included 157 (42.7%) non-neoplastic lesions, 62 (16.9%) lymphomas and 148 (40.3%) metastatic neoplasms. Cytology had a sensitivity of 66.6% [95% confidence interval (CI) 60.0-72.8%], specificity of 91.5% (CI 86.3-95.2%), and accuracy of 77.2% (CI 72.6-81.3%) for neoplasia. Likelihood of malignancy with a positive cytologic diagnosis of neoplasia was 93.0%. High proportions of false-negative results were found in mesenteric T-cell lymphoma (22/35, 63%, mainly cats), metastatic sarcoma (8/14, 57%) and metastatic mast cell tumour (15/48, 31%, mainly dogs). Factors contributing to discrepancies included well-differentiated lymphocyte morphology, focal distribution of metastases and poorly defined criteria for metastatic mast cell tumours.

Original languageEnglish (US)
JournalVeterinary and Comparative Oncology
DOIs
StateAccepted/In press - 2016

Fingerprint

Felidae
retrospective studies
lymph nodes
Canidae
Retrospective Studies
Lymph Nodes
cats
neoplasms
dogs
confidence interval
Neoplasms
mast cells
Cats
lymphoma
Dogs
Confidence Intervals
Mast Cells
sarcoma
T-Cell Lymphoma
lesions (animal)

Keywords

  • Biopsy
  • Fine-needle aspirate
  • Lymphoma
  • Metastasis
  • Small animal

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{f39ab34623984028a6e0988047549888,
title = "Cytologic-histologic concordance in the diagnosis of neoplasia in canine and feline lymph nodes: A retrospective study of 367 cases",
abstract = "Lymph nodes are frequently sampled in dogs and cats for the diagnosis of primary and metastatic neoplasia. We determined the accuracy of cytologic diagnosis in lymph nodes using histology as the gold standard. Lymph node reports (2001-2011) were retrospectively evaluated and diagnoses were categorized as neoplastic or non-neoplastic. Lymph nodes from 296 dogs and 71 cats included 157 (42.7{\%}) non-neoplastic lesions, 62 (16.9{\%}) lymphomas and 148 (40.3{\%}) metastatic neoplasms. Cytology had a sensitivity of 66.6{\%} [95{\%} confidence interval (CI) 60.0-72.8{\%}], specificity of 91.5{\%} (CI 86.3-95.2{\%}), and accuracy of 77.2{\%} (CI 72.6-81.3{\%}) for neoplasia. Likelihood of malignancy with a positive cytologic diagnosis of neoplasia was 93.0{\%}. High proportions of false-negative results were found in mesenteric T-cell lymphoma (22/35, 63{\%}, mainly cats), metastatic sarcoma (8/14, 57{\%}) and metastatic mast cell tumour (15/48, 31{\%}, mainly dogs). Factors contributing to discrepancies included well-differentiated lymphocyte morphology, focal distribution of metastases and poorly defined criteria for metastatic mast cell tumours.",
keywords = "Biopsy, Fine-needle aspirate, Lymphoma, Metastasis, Small animal",
author = "Ku, {C. K.} and Kass, {Philip H} and Christopher, {Mary M}",
year = "2016",
doi = "10.1111/vco.12256",
language = "English (US)",
journal = "Veterinary and Comparative Oncology",
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T1 - Cytologic-histologic concordance in the diagnosis of neoplasia in canine and feline lymph nodes

T2 - A retrospective study of 367 cases

AU - Ku, C. K.

AU - Kass, Philip H

AU - Christopher, Mary M

PY - 2016

Y1 - 2016

N2 - Lymph nodes are frequently sampled in dogs and cats for the diagnosis of primary and metastatic neoplasia. We determined the accuracy of cytologic diagnosis in lymph nodes using histology as the gold standard. Lymph node reports (2001-2011) were retrospectively evaluated and diagnoses were categorized as neoplastic or non-neoplastic. Lymph nodes from 296 dogs and 71 cats included 157 (42.7%) non-neoplastic lesions, 62 (16.9%) lymphomas and 148 (40.3%) metastatic neoplasms. Cytology had a sensitivity of 66.6% [95% confidence interval (CI) 60.0-72.8%], specificity of 91.5% (CI 86.3-95.2%), and accuracy of 77.2% (CI 72.6-81.3%) for neoplasia. Likelihood of malignancy with a positive cytologic diagnosis of neoplasia was 93.0%. High proportions of false-negative results were found in mesenteric T-cell lymphoma (22/35, 63%, mainly cats), metastatic sarcoma (8/14, 57%) and metastatic mast cell tumour (15/48, 31%, mainly dogs). Factors contributing to discrepancies included well-differentiated lymphocyte morphology, focal distribution of metastases and poorly defined criteria for metastatic mast cell tumours.

AB - Lymph nodes are frequently sampled in dogs and cats for the diagnosis of primary and metastatic neoplasia. We determined the accuracy of cytologic diagnosis in lymph nodes using histology as the gold standard. Lymph node reports (2001-2011) were retrospectively evaluated and diagnoses were categorized as neoplastic or non-neoplastic. Lymph nodes from 296 dogs and 71 cats included 157 (42.7%) non-neoplastic lesions, 62 (16.9%) lymphomas and 148 (40.3%) metastatic neoplasms. Cytology had a sensitivity of 66.6% [95% confidence interval (CI) 60.0-72.8%], specificity of 91.5% (CI 86.3-95.2%), and accuracy of 77.2% (CI 72.6-81.3%) for neoplasia. Likelihood of malignancy with a positive cytologic diagnosis of neoplasia was 93.0%. High proportions of false-negative results were found in mesenteric T-cell lymphoma (22/35, 63%, mainly cats), metastatic sarcoma (8/14, 57%) and metastatic mast cell tumour (15/48, 31%, mainly dogs). Factors contributing to discrepancies included well-differentiated lymphocyte morphology, focal distribution of metastases and poorly defined criteria for metastatic mast cell tumours.

KW - Biopsy

KW - Fine-needle aspirate

KW - Lymphoma

KW - Metastasis

KW - Small animal

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