Cytologic and immunocytochemical characterization of feline progressive histiocytosis

Nazaré Pinto da Cunha, Gabriele Ghisleni, Fabia Scarampella, Fabrizio Fabbrini, Monica Sforna, Luisa Cornegliani, Mario Caniatti, Giancarlo Avallone, Peter F Moore, Paola Roccabianca

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Background: Feline Progressive Histiocytosis (FPH) is a cutaneous dendritic cell neoplasm characterized by slow progression and spread to internal organs in the terminal stage. FPH is often misdiagnosed as an inflammatory reaction and has not been fully characterized from a cytologic diagnostic perspective. Objectives: The purpose of the study was to characterize the cytologic and immunocytochemical aspects useful for FPH diagnosis. Methods: Fine-needle aspiration cytologic samples of 5 cases of FPH confirmed by skin biopsy and necropsy were evaluated. Immunocytochemistry with antibodies recognizing CD1a, CD1c, CD3, CD11b, CD18, CD21, and MHCII was performed on air-dried, acetone-fixed smears. E-cadherin expression was assessed on paraffin-embedded skin biopsies. Transmission electron microscopy (TEM) was performed in one case. Results: Main cytologic findings on variably cellular samples were characterized by single to cohesive large, round to polygonal cells with intermediate to low N/C ratio, abundant clear homogeneous cytoplasm, and round to oval nuclei with rare bi- to multinucleated atypical cells, associated with low numbers of small lymphocytes and/or neutrophils. Neoplastic cells expressed CD1a, CD1c, CD11b, CD18, and MHCII. Anti-CD3 antibodies identified reactive T cells admixed with the neoplastic cells. E-cadherin expression was observed in all but one case. TEM failed to identify Birbeck granules in one case. Conclusions: FPH is a distinctive neoplastic lesion composed of nonphagocytizing histiocytes variably admixed with neutrophils and small mature lymphocytes. Immunocytochemical analysis with CD1 is mandatory to confirm a dendritic cell origin. Immunocytochemistry and cytomorphology allowed the specific and rapid diagnosis of FPH on cytologic samples.

Original languageEnglish (US)
Pages (from-to)428-436
Number of pages9
JournalVeterinary Clinical Pathology
Issue number3
StatePublished - 2014


  • Cats
  • CD1
  • Histiocytic disorders
  • Immunocytochemistry
  • Skin

ASJC Scopus subject areas

  • veterinary(all)


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