Cytokines gene expression patterns of bovine milk during middle and late stages of lactation.

A. M. Alluwaimi, J. S. Cullor

Research output: Contribution to journalArticle

Abstract

The cytokine mRNA profiles of the bovine mammary gland were investigated using newly developed TaqMan real-time polymerase chain reaction systems (Applied Biosystems, Foster City, CA, USA). Transcriptional activity of six cytokines, interleukin (IL)-2, IL-6, IL-12, interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha and granulocyte-macrophage colony stimulating factor (GM-CSF) was studied during the mid- and late-lactation stages. Transcripts for TNF-alpha, GM-CSF, and IFN-gamma were detected in all samples of both stages. However, IL-12 was only detected in 80 and 58 % of late- and mid-lactation samples, respectively. IL-12 expression was up-regulated in late lactation in comparison with the corresponding level in mid-lactation. The cytokines interaction in late lactation was more co-ordinated and their transcriptional levels were significantly correlated among each other, whereas, in mid-lactation significant correlation of the cytokines transcription was only seen with the TNF-alpha, GM-CSF, and IFN-gamma. Cytokine mRNA profiles between mid- and late lactation showed significant differences, which can be attributed to the dramatic changes that the mammary gland is subjected to during late lactation. The significant elevation of IL-12 transcriptional activity in late lactation and its relevance to the mammary gland immunity is discussed.

Original languageEnglish (US)
JournalJournal of veterinary medicine. B, Infectious diseases and veterinary public health
Volume49
Issue number2
StatePublished - Mar 2002

Fingerprint

late lactation
lactation stage
Lactation
Milk
cytokines
interleukin-12
Cytokines
granulocyte-macrophage colony-stimulating factor
Gene Expression
milk
gene expression
interferon-gamma
mammary glands
Interleukin-12
tumor necrosis factor-alpha
lactation
Human Mammary Glands
Granulocyte-Macrophage Colony-Stimulating Factor
Interferon-gamma
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)
  • Microbiology

Cite this

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title = "Cytokines gene expression patterns of bovine milk during middle and late stages of lactation.",
abstract = "The cytokine mRNA profiles of the bovine mammary gland were investigated using newly developed TaqMan real-time polymerase chain reaction systems (Applied Biosystems, Foster City, CA, USA). Transcriptional activity of six cytokines, interleukin (IL)-2, IL-6, IL-12, interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha and granulocyte-macrophage colony stimulating factor (GM-CSF) was studied during the mid- and late-lactation stages. Transcripts for TNF-alpha, GM-CSF, and IFN-gamma were detected in all samples of both stages. However, IL-12 was only detected in 80 and 58 {\%} of late- and mid-lactation samples, respectively. IL-12 expression was up-regulated in late lactation in comparison with the corresponding level in mid-lactation. The cytokines interaction in late lactation was more co-ordinated and their transcriptional levels were significantly correlated among each other, whereas, in mid-lactation significant correlation of the cytokines transcription was only seen with the TNF-alpha, GM-CSF, and IFN-gamma. Cytokine mRNA profiles between mid- and late lactation showed significant differences, which can be attributed to the dramatic changes that the mammary gland is subjected to during late lactation. The significant elevation of IL-12 transcriptional activity in late lactation and its relevance to the mammary gland immunity is discussed.",
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