Cytokine transcription in lymph nodes of cattle in different stages of bovine leukemia virus infection

Robert G. Keefe, David A. Ferrick, Jeffrey L Stott

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Bovine leukemia virus (BLV) is a transforming oncovirus that contains no oncogenes or preferred site of proviral integration. The role of cytokines in the disease process of BLV is potentially important due to the similarity of BLV with other retroviruses in which cytokines play a role, such as HTLV-I and -II. Mesenteric and supra-mammary lymph nodes were obtained from a panel of nine cattle. Three were non-infected controls, three were BLV-positive aleukemic (AL), and three were BLV-positive persistent lymphocytotic (PL). Mononuclear cells were perfused from the organs and total RNA extracted from either 1 x 108 unseparated cells or 1 x 107 purified CD4/CD8 T-cells. cDNA was generated and subjected to RT-PCR to analyze cytokine transcription during disease progression. cDNA levels were normalized using β-actin PCR at sub-plateau cycle number, enabling a semi-quantitative assessment of cytokine gene transcripts. Using this approach, IL-2, IL-10 and IFN-γ message was detected in the T-cell fractions of all of the BLV-infected animals, but not in the non-infected controls.

Original languageEnglish (US)
Pages (from-to)271-283
Number of pages13
JournalVeterinary Immunology and Immunopathology
Volume59
Issue number3-4
DOIs
StatePublished - Nov 1997

Fingerprint

Bovine Leukemia Virus
Bovine leukemia virus
Virus Diseases
lymph nodes
cytokines
transcription (genetics)
Lymph Nodes
Cytokines
cattle
infection
Retroviridae
Complementary DNA
T-lymphocytes
Betaretrovirus
Human T-lymphotropic virus 2
T-Lymphocytes
Polymerase Chain Reaction
Human T-lymphotropic virus 1
oncogenes
interleukin-10

Keywords

  • BLV
  • Bovine
  • Cytokine
  • Retrovirus
  • RT-PCR
  • T-cells

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Cytokine transcription in lymph nodes of cattle in different stages of bovine leukemia virus infection. / Keefe, Robert G.; Ferrick, David A.; Stott, Jeffrey L.

In: Veterinary Immunology and Immunopathology, Vol. 59, No. 3-4, 11.1997, p. 271-283.

Research output: Contribution to journalArticle

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