Cytokine production in patients with anorexia nervosa, bulimia nervosa and obesity

Nancy C. Raymond, Maurice Dysken, Kris Bettin, Elke D. Eckert, Scott J. Crow, Kristy Markus, Claire Pomeroy

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Objective: We previously reported elevated serum levels of the cytokines interleukin-6 (IL-6) and transforming growth factor-β (TGF-β) in patients with anorexia nervosa (AN). We investigated the cellular production of these two cytokines and of interferon-γ (IFN-γ), interleukin-1α (IL-1α), and tumor necrosis factor-α (TNF-α) in subjects with AN, bulimia nervosa (BN), and obesity as well as in normal-weight control subjects. Methods: Supernatant fluids from isolated peripheral blood mononuclear cells (PBMC) incubated with and without concanavalin A (ConA) were assayed for cytokine concentrations by enzyme-linked immunosorbent assay (ELISA). Results: Significant differences across the four groups were found in the stimulated cellular production of IFN-γ and IL-6. Stimulated IFN-γ production was elevated in the AN group compared to controls. IL-6 production was significantly elevated in obese subjects relative to the two normal-weight groups, BN and controls, and tended to be higher in the AN group than in the controls, but not significantly so. IL-1α production was greater in obese subjects. Conclusion: The findings of increased IFN-γ production and a tendency toward increased IL-6 production (both of which suppress food intake in animals) in individuals who severely restrict food intake suggest a potential role for these cytokines in the pathogenesis of AN. Elevated IL-6 and IL-1α production by PBMC in obese individuals requires further investigation to determine if these cytokines contribute to the development or perpetuation of obesity. (C) 2000 by John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)293-302
Number of pages10
JournalInternational Journal of Eating Disorders
Volume28
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Bulimia Nervosa
anorexia nervosa
Anorexia Nervosa
interleukin-6
interferons
Interleukin-6
obesity
cytokines
Obesity
Interferons
Cytokines
interleukin-1
Interleukin-1
mononuclear leukocytes
food intake
Blood Cells
Eating
Weights and Measures
transforming growth factors
tumor necrosis factors

Keywords

  • Anorexia nervosa
  • Bulimia nervosa
  • Cytokines
  • Immunology
  • Mononuclear cells
  • Obesity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Food Science
  • Psychology(all)
  • Clinical Psychology

Cite this

Cytokine production in patients with anorexia nervosa, bulimia nervosa and obesity. / Raymond, Nancy C.; Dysken, Maurice; Bettin, Kris; Eckert, Elke D.; Crow, Scott J.; Markus, Kristy; Pomeroy, Claire.

In: International Journal of Eating Disorders, Vol. 28, No. 3, 2000, p. 293-302.

Research output: Contribution to journalArticle

Raymond, Nancy C. ; Dysken, Maurice ; Bettin, Kris ; Eckert, Elke D. ; Crow, Scott J. ; Markus, Kristy ; Pomeroy, Claire. / Cytokine production in patients with anorexia nervosa, bulimia nervosa and obesity. In: International Journal of Eating Disorders. 2000 ; Vol. 28, No. 3. pp. 293-302.
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AU - Raymond, Nancy C.

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AU - Eckert, Elke D.

AU - Crow, Scott J.

AU - Markus, Kristy

AU - Pomeroy, Claire

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N2 - Objective: We previously reported elevated serum levels of the cytokines interleukin-6 (IL-6) and transforming growth factor-β (TGF-β) in patients with anorexia nervosa (AN). We investigated the cellular production of these two cytokines and of interferon-γ (IFN-γ), interleukin-1α (IL-1α), and tumor necrosis factor-α (TNF-α) in subjects with AN, bulimia nervosa (BN), and obesity as well as in normal-weight control subjects. Methods: Supernatant fluids from isolated peripheral blood mononuclear cells (PBMC) incubated with and without concanavalin A (ConA) were assayed for cytokine concentrations by enzyme-linked immunosorbent assay (ELISA). Results: Significant differences across the four groups were found in the stimulated cellular production of IFN-γ and IL-6. Stimulated IFN-γ production was elevated in the AN group compared to controls. IL-6 production was significantly elevated in obese subjects relative to the two normal-weight groups, BN and controls, and tended to be higher in the AN group than in the controls, but not significantly so. IL-1α production was greater in obese subjects. Conclusion: The findings of increased IFN-γ production and a tendency toward increased IL-6 production (both of which suppress food intake in animals) in individuals who severely restrict food intake suggest a potential role for these cytokines in the pathogenesis of AN. Elevated IL-6 and IL-1α production by PBMC in obese individuals requires further investigation to determine if these cytokines contribute to the development or perpetuation of obesity. (C) 2000 by John Wiley and Sons, Inc.

AB - Objective: We previously reported elevated serum levels of the cytokines interleukin-6 (IL-6) and transforming growth factor-β (TGF-β) in patients with anorexia nervosa (AN). We investigated the cellular production of these two cytokines and of interferon-γ (IFN-γ), interleukin-1α (IL-1α), and tumor necrosis factor-α (TNF-α) in subjects with AN, bulimia nervosa (BN), and obesity as well as in normal-weight control subjects. Methods: Supernatant fluids from isolated peripheral blood mononuclear cells (PBMC) incubated with and without concanavalin A (ConA) were assayed for cytokine concentrations by enzyme-linked immunosorbent assay (ELISA). Results: Significant differences across the four groups were found in the stimulated cellular production of IFN-γ and IL-6. Stimulated IFN-γ production was elevated in the AN group compared to controls. IL-6 production was significantly elevated in obese subjects relative to the two normal-weight groups, BN and controls, and tended to be higher in the AN group than in the controls, but not significantly so. IL-1α production was greater in obese subjects. Conclusion: The findings of increased IFN-γ production and a tendency toward increased IL-6 production (both of which suppress food intake in animals) in individuals who severely restrict food intake suggest a potential role for these cytokines in the pathogenesis of AN. Elevated IL-6 and IL-1α production by PBMC in obese individuals requires further investigation to determine if these cytokines contribute to the development or perpetuation of obesity. (C) 2000 by John Wiley and Sons, Inc.

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