Cytokine production by rabbit alveolar macrophages: Differences between activated and suppressor cell phenotypes

Hiroyuki Kobayashi, Makiko Kobayashi, Thomas A. Heming, Akhil Bidani, Richard B Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The differences between cytokine-producing profiles of activated macrophages (A-Mφ) and suppressor macrophages (S-Mφ) were examined. A-Mφ, which exhibited cytotoxicity against RK-13 cells, were generated from resident rabbit alveolar Mφ by treatment with lymphokine solution (culture fluids of rabbit spleen cells stimulated with concanavalin A [Con A]). S-Mφ, which were able to inhibit cellular proliferations of rabbit spleen cells stimulated with Con A, were generated from resident alveolar Mφ by treatment with 1-methyladenosine (an immunosuppressive molecule in tumourous ascites fluids). When A-Mφ were stimulated with lipopolysaccharide (LPS) in vitro, the cells produced significantly more interleukin (IL)-1 (~1.4 times), IL-6 (~2.1 times), IL-12 (~60 times), and tumour necrosis factor-α (TNF-α) (~37 times) than did resting macrophages (R-Mφ) stimulated with LPS as control cells. After the stimulation with LPS, both A-Mφ and R-Mφ did not produce transforming growth factor-β (TGF-β). In contrast, when S-Mφ were stimulated with LPS in vitro, the cells produced significantly more TGF-β (~1.6 times) and significantly less IL-6 (~1.8 times) than did control cells. Also, S-Mφ did not produce IL-1, IL-12, and TNF-α into their culture fluids after the stimulation with LPS. These results show the differences between cytokine-producing profiles of A-Mφ and S-Mφ and characteristics of their cytokine-producing profiles are analogous to T cell subsets. Differences displayed in the cytokine profiles may contribute to the effector (A-Mφ) or the suppressor (S-Mφ) functions of alveolar Mφ.

Original languageEnglish (US)
Pages (from-to)339-346
Number of pages8
JournalImmunology Letters
Volume69
Issue number3
DOIs
StatePublished - Sep 1 1999
Externally publishedYes

Fingerprint

Alveolar Macrophages
Macrophages
Cytokines
Rabbits
Phenotype
Lipopolysaccharides
Transforming Growth Factors
Interleukin-12
Concanavalin A
Interleukin-1
Interleukin-6
Spleen
Tumor Necrosis Factor-alpha
Lymphokines
T-Lymphocyte Subsets
Immunosuppressive Agents
Ascites
Cell Proliferation

Keywords

  • Alveolar macrophages
  • Classification
  • Cytokine-producing profiles

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Cytokine production by rabbit alveolar macrophages : Differences between activated and suppressor cell phenotypes. / Kobayashi, Hiroyuki; Kobayashi, Makiko; Heming, Thomas A.; Bidani, Akhil; Pollard, Richard B; Suzuki, Fujio.

In: Immunology Letters, Vol. 69, No. 3, 01.09.1999, p. 339-346.

Research output: Contribution to journalArticle

Kobayashi, Hiroyuki ; Kobayashi, Makiko ; Heming, Thomas A. ; Bidani, Akhil ; Pollard, Richard B ; Suzuki, Fujio. / Cytokine production by rabbit alveolar macrophages : Differences between activated and suppressor cell phenotypes. In: Immunology Letters. 1999 ; Vol. 69, No. 3. pp. 339-346.
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