Cytokeratin-filament expression in epithelial and non-epithelial tissues of the common carp (Cyprinus carpio)

Joseph M. Groff, Diane K. Naydan, Robert Higgins, Joseph G. Zinkl

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Cytokeratin expression in mammals is generally restricted to epithelial cells and has been utilized to differentiate epithelial from nonepithelial tissues in these species. Since cytokeratins have been shown to be highly conserved during vertebrate evolution, the objective of the present study has been to ascertain the expression pattern of cytokeratins in tissues of the common carp (Cyprinus carpio). A panel of 10 anti-human cytokeratin antibodies was evaluated using a streptavidin-biotin-peroxidase complex detection system. Tissues were fixed in 10% neutral-buffered formalin, 100% ethanol or methacarn. Only formalin-fixed tissues were pre-digested with trypsin prior to immunostaining. Formalin-fixed tissues generally resulted in a less intense, more diffuse staining pattern with considerable background compared with ethanol and methacarn and was therefore the least desirable fixative. The diverse staining pattern observed with the various antibodies used in this study was consistent with previous findings in other teleosts. The results confirm that cytokeratin expression in teleosts is fundamentally different from that in mammals and therefore should be used as a method to differentiate epithelial cell types in these species only with discretion.

Original languageEnglish (US)
Pages (from-to)375-384
Number of pages10
JournalCell and Tissue Research
Volume287
Issue number2
DOIs
StatePublished - Jan 1 1997

Fingerprint

Carps
Keratins
Formaldehyde
Mammals
Ethanol
Epithelial Cells
Staining and Labeling
Fixatives
Antibodies
Trypsin
Peroxidase
Vertebrates
Epithelium

Keywords

  • Cyprinus carpio (Teleostei)
  • Cytokeratins
  • Cytoskeleton
  • Epithelia
  • Immunohistochemistry
  • Intermediate filaments
  • Mesenchyme
  • Tissue fixation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Cytokeratin-filament expression in epithelial and non-epithelial tissues of the common carp (Cyprinus carpio). / Groff, Joseph M.; Naydan, Diane K.; Higgins, Robert; Zinkl, Joseph G.

In: Cell and Tissue Research, Vol. 287, No. 2, 01.01.1997, p. 375-384.

Research output: Contribution to journalArticle

Groff, Joseph M. ; Naydan, Diane K. ; Higgins, Robert ; Zinkl, Joseph G. / Cytokeratin-filament expression in epithelial and non-epithelial tissues of the common carp (Cyprinus carpio). In: Cell and Tissue Research. 1997 ; Vol. 287, No. 2. pp. 375-384.
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