Cytocidal etfect of high energy shock wave on tumour cells enhanced with larger dose and multiple exposures

C. Z. Yao, J. Ishizuka, Richard J Bold, C. M. Townsend, J. C. Thompson

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Cultured LLC-WRC256 (Walker rat carcinoma) cells were exposed to different doses of high energy shock waves (HESW). The immediate viabilities were 98% in the control cells, and 74%, 53% and 18% following 400, 800, and 1500 HESW treatment, respectively. Surviving cells in the 400 and 800-treated HESW demonstrated delayed upward growth rate curves, and the 1500 HESW-treated a downward curve. Agar clonogenic etficiencies for surviving cells were 36% (control), 20% (400 HESW), 15% (800 HESW) and 3% (1500 HESW). LLC-WRC256 tumours in Wistar rats were treated once every other day with 1500 HESW on a total of three occasions. Tumours treated with HESW grew more slowly (4.9 cm3) than those in the control (13.5 cm3). HESW fragmented cells and destroyed cell membranes and intracellular organelles. A histological examination of tumours treated with HESW demonstrated local haemorrhage with necrosis in the HESW focus area. Damage to the surrounding skin and soft tissue was slight and transient. These findings suggest that the growth of tumour cells can be suppressed in vitro and in vivo by treatment with HESW.

Original languageEnglish (US)
Pages (from-to)229-235
Number of pages7
JournalSurgical Oncology
Volume3
Issue number4
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • clonogenic efficiency
  • high energy shock wave (HESW)
  • LLC-WRC 256

ASJC Scopus subject areas

  • Oncology
  • Surgery

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