@article{6e8f121c10804ba1bd8d1ed6c8d8189a,
title = "Cytochrome P450-catalyzed biosynthesis of furanoditerpenoids in the bioenergy crop switchgrass (Panicum virgatum L.)",
abstract = "Specialized diterpenoid metabolites are important mediators of plant–environment interactions in monocot crops. To understand metabolite functions in plant environmental adaptation that ultimately can enable crop improvement strategies, a deeper knowledge of the underlying species-specific biosynthetic pathways is required. Here, we report the genomics-enabled discovery of five cytochrome P450 monooxygenases (CYP71Z25–CYP71Z29) that form previously unknown furanoditerpenoids in the monocot bioenergy crop Panicum virgatum (switchgrass). Combinatorial pathway reconstruction showed that CYP71Z25–CYP71Z29 catalyze furan ring addition directly to primary diterpene alcohol intermediates derived from distinct class II diterpene synthase products. Transcriptional co-expression patterns and the presence of select diterpenoids in switchgrass roots support the occurrence of P450-derived furanoditerpenoids in planta. Integrating molecular dynamics, structural analysis and targeted mutagenesis identified active site determinants that contribute to the distinct catalytic specificities underlying the broad substrate promiscuity of CYP71Z25–CYP71Z29 for native and non-native diterpenoids.",
keywords = "cytochrome P450 monooxygenase, diterpenoid biosynthesis, Panicum virgatum, plant natural products, plant specialized metabolism",
author = "Andrew Muchlinski and Meirong Jia and Kira Tiedge and Fell, {Jason S.} and Pelot, {Kyle A.} and Lisl Chew and Danielle Davisson and Yuxuan Chen and Justin Siegel and Lovell, {John T.} and Philipp Zerbe",
note = "Funding Information: We gratefully acknowledge the US Department of Energy Joint Genome Institute (DOE JGI) and collaborators for pre‐publication access to the v1.1 and v4.1 genome sequence data produced by DOE JGI. We further thank Dr Reuben Peters (Iowa State University, Ames, IA, USA) for providing the pIRS and pGGxC constructs, Dr Dean Tantillo (University of California – Davis, Davis, CA, USA) for helpful discussions on the CYP71Z25–CYP71Z29 catalytic mechanism, the UC Davis Genome Center Bioinformatics Core for providing high‐performance computing resources, Dr Malay Saha (Nobel Research Institute, Ardmore, OK, USA) for providing tillers of var. Alamo AP13. Financial support for this work was provided by the US Department of Energy (DOE) Early Career Research Program (DE‐SC0019178, to PZ) and a DOE Joint Genome Institute (JGI) DNA Synthesis Science Program (grant no. 2568, to PZ). The gene synthesis work conducted by the US Department of Energy Joint Genome Institute (JGI), a DOE Office of Science User Facility, is supported by the Office of Science of the US Department of Energy under contract no. DE‐AC02‐05CH11231. Panicum virgatum P. virgatum Publisher Copyright: {\textcopyright} 2021 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.",
year = "2021",
doi = "10.1111/tpj.15492",
language = "English (US)",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
}