Cystic fibrosis decreases the apical membrane chloride permeability of monolayers cultured from cells of tracheal epithelium

Jonathan Widdicombe, M. J. Welsh, W. E. Finkbeiner

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Abstract

The tracheal mucosa from a 12-year-old girl was digested with collagenase 4 hr after her death from cystic fibrosis. Forty million viable cells were obtained. The cells, plated at 106 per cm2 onto four Nuclepore filters coated with human placental collagen, formed confluent monolayers after 1 day. Their ultrastructure was similar to that of normal human cells. They were studied in conventional Ussing chambers or with intracellular microelectrodes on days 5-7 after plating. The monolayers displayed resistance of 380 ± 50 Ω.cm2 and short-circuit current (I(sc)) of 1.8 ± 0.4 μA.cm-2. This resistance is similar to that obtained for dog or normal human monolayers. The I(sc) is less than normal human (≃3 μA.cm-2) or dog (≃10 μA.cm-2) cells. The cystic fibrosis cells resembled normal monolayers in that serosal ouabain and mucosal amiloride inhibited I(sc), while mucosal ouabain or serosal amiloride had no effect. They differed from normal human or dog cells in that I(sc) was not inhibited by bumetanide and the stimulation of I(sc) by isoproterenol or prostaglandin E2 was greatly reduced or abolished. Addition of isoproterenol depolarized apical membrane potential (ψ(a)) and decreased fractional resistance (f(R)) in normal human and dog but had no effect on ψ(a) or f(R) in cystic fibrosis cells. Reduction of mucosal chloride from 120 to 5 mM by replacement with gluconate increased f(R) of dog and normal human monolayers and depolarized ψ(a) by 22 (dog) or 30 (human) mV. In cystic fibrosis monolayers, chloride replacement hyperpolarized ψ(a) by 2 mV and had little effect on f(R). These results suggest that the primary defect in cystic fibrosis is reduced apical membrane chloride conductance.

Original languageEnglish (US)
Pages (from-to)6167-6171
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number18
StatePublished - 1985
Externally publishedYes

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ASJC Scopus subject areas

  • General
  • Genetics

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