Cyclooxygenase-2 mRNA expression is associated with c-fos mRNA expression and transient water ADC reduction detected with diffusion MRI during acute focal ischemia in rats

Anthony Mancuso, Nikita Derugin, Kazushi Hara, Theresa A. Marsh, Daniel Kong, Frank R Sharp, Philip R. Weinstein

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cyclooxygenase-2 (COX-2) plays an important role in the development of injury during cerebral ischemia and inhibition of its activity can reduce infarct size. COX-2 expression during acute ischemia is caused by activation of post-synaptic glutamate receptors, which occurs during spreading depression and ischemic depolarization. Both of these phenomena cause a reduction in the apparent diffusion coefficient of water (ADC), which can be detected with diffusion-weighted magnetic resonance imaging. The reduction is believed to be caused by cellular swelling that occurs as cells depolarize. The goal of this work was to determine the spatial relationship between cyclooxygenase-2 mRNA (cox-2) expression, c-fos mRNA expression and ADC reduction during acute focal cerebral ischemia. Adult rats were subjected to either 30- or 60-min permanent occlusion of the middle cerebral artery. A 2-Tesla scanner was used to acquire diffusion-weighted echo-planar images throughout the ischemic period, which were used to calculate ADC maps. Cox-2 and c-fos mRNA were detected with 35S in situ hybridization. The results indicate that, for rats subjected to 60-min ischemia, cox-2 was observed in superficial layers of cortex, where transient ADC reduction and c-fos expression were observed. The same was true for most rats subjected to 30-min ischemia. However, in a small number of rats of the 30-min group, cox-2 mRNA expression was observed in regions exhibiting transient and persistent ADC reduction with no c-fos expression. The results suggest that cox-2 mRNA expression during acute MCA occlusion is caused by either or both spreading depression and transient ischemic depolarization.

Original languageEnglish (US)
Pages (from-to)121-130
Number of pages10
JournalBrain Research
Volume961
Issue number1
DOIs
StatePublished - Jan 24 2003
Externally publishedYes

Fingerprint

Diffusion Magnetic Resonance Imaging
Cyclooxygenase 2
Ischemia
Messenger RNA
Water
Brain Ischemia
Neurotransmitter Receptor
Middle Cerebral Artery Infarction
Glutamate Receptors
In Situ Hybridization
Wounds and Injuries

Keywords

  • c-fos
  • Cox-2
  • Diffusion MRI
  • Echo-planar imaging
  • Focal cerebral ischemia
  • Immediate early gene

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cyclooxygenase-2 mRNA expression is associated with c-fos mRNA expression and transient water ADC reduction detected with diffusion MRI during acute focal ischemia in rats. / Mancuso, Anthony; Derugin, Nikita; Hara, Kazushi; Marsh, Theresa A.; Kong, Daniel; Sharp, Frank R; Weinstein, Philip R.

In: Brain Research, Vol. 961, No. 1, 24.01.2003, p. 121-130.

Research output: Contribution to journalArticle

Mancuso, Anthony ; Derugin, Nikita ; Hara, Kazushi ; Marsh, Theresa A. ; Kong, Daniel ; Sharp, Frank R ; Weinstein, Philip R. / Cyclooxygenase-2 mRNA expression is associated with c-fos mRNA expression and transient water ADC reduction detected with diffusion MRI during acute focal ischemia in rats. In: Brain Research. 2003 ; Vol. 961, No. 1. pp. 121-130.
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