Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene

J. D. Regan; L. H. Thompson; W. L. Carrier; C. A. Weber; A. A. Francis; M. Z. Zdzienicka

doi:10.1016/0921-8777(90)90069-H

Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene

J. D. Regan, L. H. Thompson, W. L. Carrier, C. A. Weber, A. A. Francis, M. Z. Zdzienicka

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.

Original language	English (US)
Pages (from-to)	157-163
Number of pages	7
Journal	Mutation Research-DNA Repair
Volume	235
Issue number	3
DOIs	https://doi.org/10.1016/0921-8777(90)90069-H
State	Published - 1990
Externally published	Yes

Keywords

Cyclobutane-pyrimidine dimer excision
DNA nucleotide excision-repair mutants
ERCC2 repair gene

ASJC Scopus subject areas

Genetics
Molecular Biology
Toxicology
Medicine(all)

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title = "Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene",

abstract = "Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.",

keywords = "Cyclobutane-pyrimidine dimer excision, DNA nucleotide excision-repair mutants, ERCC2 repair gene",

author = "Regan, {J. D.} and Thompson, {L. H.} and Carrier, {W. L.} and Weber, {C. A.} and Francis, {A. A.} and Zdzienicka, {M. Z.}",

year = "1990",

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T1 - Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene

AU - Regan, J. D.

AU - Thompson, L. H.

AU - Carrier, W. L.

AU - Weber, C. A.

AU - Francis, A. A.

AU - Zdzienicka, M. Z.

PY - 1990

Y1 - 1990

N2 - Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.

AB - Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.

KW - Cyclobutane-pyrimidine dimer excision

KW - DNA nucleotide excision-repair mutants

KW - ERCC2 repair gene

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