Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene

J. D. Regan, L. H. Thompson, W. L. Carrier, C. A. Weber, A. A. Francis, M. Z. Zdzienicka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.

Original languageEnglish (US)
Pages (from-to)157-163
Number of pages7
JournalMutation Research-DNA Repair
Volume235
Issue number3
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Pyrimidine Dimers
Dimers
Repair
Genes
Cell Line
CHO Cells
Cells
Cricetulus
DNA Repair
Cricetinae
Ovary
Rodentia
Assays
Nucleotides
DNA
Irradiation

Keywords

  • Cyclobutane-pyrimidine dimer excision
  • DNA nucleotide excision-repair mutants
  • ERCC2 repair gene

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Toxicology
  • Medicine(all)

Cite this

Regan, J. D., Thompson, L. H., Carrier, W. L., Weber, C. A., Francis, A. A., & Zdzienicka, M. Z. (1990). Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene. Mutation Research-DNA Repair, 235(3), 157-163. https://doi.org/10.1016/0921-8777(90)90069-H

Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene. / Regan, J. D.; Thompson, L. H.; Carrier, W. L.; Weber, C. A.; Francis, A. A.; Zdzienicka, M. Z.

In: Mutation Research-DNA Repair, Vol. 235, No. 3, 1990, p. 157-163.

Research output: Contribution to journalArticle

Regan, J. D. ; Thompson, L. H. ; Carrier, W. L. ; Weber, C. A. ; Francis, A. A. ; Zdzienicka, M. Z. / Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene. In: Mutation Research-DNA Repair. 1990 ; Vol. 235, No. 3. pp. 157-163.
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