Abstract
Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.
Original language | English (US) |
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Pages (from-to) | 157-163 |
Number of pages | 7 |
Journal | Mutation Research-DNA Repair |
Volume | 235 |
Issue number | 3 |
DOIs | |
State | Published - 1990 |
Externally published | Yes |
Keywords
- Cyclobutane-pyrimidine dimer excision
- DNA nucleotide excision-repair mutants
- ERCC2 repair gene
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Toxicology
- Medicine(all)