Abstract
Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 157-163 |
| Number of pages | 7 |
| Journal | Mutation Research-DNA Repair |
| Volume | 235 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1990 |
| Externally published | Yes |
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Keywords
- Cyclobutane-pyrimidine dimer excision
- DNA nucleotide excision-repair mutants
- ERCC2 repair gene
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Toxicology
- Medicine(all)
Cite this
Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene. / Regan, J. D.; Thompson, L. H.; Carrier, W. L.; Weber, C. A.; Francis, A. A.; Zdzienicka, M. Z.
In: Mutation Research-DNA Repair, Vol. 235, No. 3, 1990, p. 157-163.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene
AU - Regan, J. D.
AU - Thompson, L. H.
AU - Carrier, W. L.
AU - Weber, C. A.
AU - Francis, A. A.
AU - Zdzienicka, M. Z.
PY - 1990
Y1 - 1990
N2 - Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.
AB - Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.
KW - Cyclobutane-pyrimidine dimer excision
KW - DNA nucleotide excision-repair mutants
KW - ERCC2 repair gene
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UR - http://www.scopus.com/inward/citedby.url?scp=0025257316&partnerID=8YFLogxK
U2 - 10.1016/0921-8777(90)90069-H
DO - 10.1016/0921-8777(90)90069-H
M3 - Article
C2 - 2342503
AN - SCOPUS:0025257316
VL - 235
SP - 157
EP - 163
JO - Mutation Research - DNA Repair
JF - Mutation Research - DNA Repair
SN - 0921-8777
IS - 3
ER -