Cycling of NMDA receptors during trafficking in neurons before synapse formation

Philip Washbourne, Xiao Bo Liu, Edward G. Jones, A Kimberley Usrey

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The trafficking of glutamate receptors in neurons is of the utmost importance for synapse formation and synaptic plasticity. Recently, we demonstrated that both NMDA and AMPA receptors reside in mobile transport packets that are recruited rapidly and independently to nascent synapses. Here, we show that a large proportion of the glutamate receptor clusters in young cortical neurons are present on the surface of dendrites before synapses are formed and these surface-exposed transport packets are mobile. Exocytosis of glutamate receptors to the dendritic surface occurs via a SNARE [soluble n-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor]-dependent SNAP-23-mediated mechanism. Endocytosis occurs rapidly after surface exposure; >50% of surface-labeled NMDA receptors (NMDARs) are endocytosed within 5 min. NMDARs are transported along microtubules on large tubulovesicular organelles, as indicated by immunoelectron microscopy, and are associated with EEA1 (early endosomal antigen 1) and SAP102 (synapse-associated protein 102), as indicated by immunocytochemistry. Most surprisingly, a large proportion of these transport packets cycle through the dendritic plasma membrane before synapse formation. These results suggest a novel model in which NMDARs cycle with the plasma membrane during pauses of movement along microtubules while trafficking.

Original languageEnglish (US)
Pages (from-to)8253-8264
Number of pages12
JournalJournal of Neuroscience
Volume24
Issue number38
DOIs
StatePublished - Sep 22 2004

Fingerprint

N-Methyl-D-Aspartate Receptors
Synapses
Glutamate Receptors
Neurons
Ethylmaleimide
Endocytosis
Microtubules
Cell Membrane
SNARE Proteins
Proteins
Neuronal Plasticity
AMPA Receptors
Immunoelectron Microscopy
Exocytosis
Dendrites
Organelles
Immunohistochemistry
Antigens

Keywords

  • Cortex
  • Dendrite
  • Development
  • Glutamate
  • Synaptogenesis
  • Transport

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cycling of NMDA receptors during trafficking in neurons before synapse formation. / Washbourne, Philip; Liu, Xiao Bo; Jones, Edward G.; Usrey, A Kimberley.

In: Journal of Neuroscience, Vol. 24, No. 38, 22.09.2004, p. 8253-8264.

Research output: Contribution to journalArticle

Washbourne, Philip ; Liu, Xiao Bo ; Jones, Edward G. ; Usrey, A Kimberley. / Cycling of NMDA receptors during trafficking in neurons before synapse formation. In: Journal of Neuroscience. 2004 ; Vol. 24, No. 38. pp. 8253-8264.
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