Cyclin G2 expression during growth inhibition

M. C. Home, M. P. Mulheisen, K. Donaldson, G. L. Goolsby, D. Tran, A. Wahl, Johannes W Hell

Research output: Contribution to journalArticlepeer-review


We isolated and characterized cDXAs encoding murine cyclm (12 and examined the cell cycle dependent expression of cyclin (1'2 mRNA in marine H cell;-. We investigated the effect of various positive and negative stimuli on the expression of cyclin G2 transcripts in different maturation stage-specific innrine B cell lines. Upon treatment of B cells with the negative growth factor TGF-Jl or growth inhibiting corticostoroids. cyclin (12 m UN A levels are increased 10-11 fold. Although untreated immature B cells (WKHI-2;il and CHlil ) and B cell receptor (BCR} cross-linked or phorbol ester plus ionoinycin stimulated mature B cells (BAL-17 and CH 1'2} proliferate and express low levels of cyclin CI2 mRNA relative to cyclin 1)2. similarly stimulated immature B cells undergo growth arrest and apoptosis concurrent with an approximate ](} fold increase in cyclin G2 transcripts and decrease in cylin 1)2 niRNAs. In contract. BCR stimulated WKHI-231 phosphoinositide signaling mutants re sis-tani to BCR induced growth arrest did not display an increase in cyclin (12 or a decrease in cyclin 1)2 mRNAs. Costirnulation ol BCR challenged immature B cells with LPS or gp39 blocked cyclin G2 rnRXA upregulation and ihe concomitant growth arrest response. Rat cyclui (12 and cyclin I ha.s recently been cloned from a rat brain c.DNA library allowing an examination of its ex pression pattern in neuronal eell lines and tissues undergoing differentiation, growth arrest diid apoptosis.

Original languageEnglish (US)
JournalFASEB Journal
Issue number9
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology


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