Abstract
We isolated and characterized cDXAs encoding murine cyclm (12 and examined the cell cycle dependent expression of cyclin (1'2 mRNA in marine H cell;-. We investigated the effect of various positive and negative stimuli on the expression of cyclin G2 transcripts in different maturation stage-specific innrine B cell lines. Upon treatment of B cells with the negative growth factor TGF-Jl or growth inhibiting corticostoroids. cyclin (12 m UN A levels are increased 10-11 fold. Although untreated immature B cells (WKHI-2;il and CHlil ) and B cell receptor (BCR} cross-linked or phorbol ester plus ionoinycin stimulated mature B cells (BAL-17 and CH 1'2} proliferate and express low levels of cyclin CI2 mRNA relative to cyclin 1)2. similarly stimulated immature B cells undergo growth arrest and apoptosis concurrent with an approximate ](} fold increase in cyclin G2 transcripts and decrease in cylin 1)2 niRNAs. In contract. BCR stimulated WKHI-231 phosphoinositide signaling mutants re sis-tani to BCR induced growth arrest did not display an increase in cyclin (12 or a decrease in cyclin 1)2 mRNAs. Costirnulation ol BCR challenged immature B cells with LPS or gp39 blocked cyclin G2 rnRXA upregulation and ihe concomitant growth arrest response. Rat cyclui (12 and cyclin I ha.s recently been cloned from a rat brain c.DNA library allowing an examination of its ex pression pattern in neuronal eell lines and tissues undergoing differentiation, growth arrest diid apoptosis.
| Original language | English (US) |
|---|---|
| Journal | FASEB Journal |
| Volume | 11 |
| Issue number | 9 |
| State | Published - 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
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Cyclin G2 expression during growth inhibition. / Home, M. C.; Mulheisen, M. P.; Donaldson, K.; Goolsby, G. L.; Tran, D.; Wahl, A.; Hell, Johannes W.
In: FASEB Journal, Vol. 11, No. 9, 1997.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cyclin G2 expression during growth inhibition
AU - Home, M. C.
AU - Mulheisen, M. P.
AU - Donaldson, K.
AU - Goolsby, G. L.
AU - Tran, D.
AU - Wahl, A.
AU - Hell, Johannes W
PY - 1997
Y1 - 1997
N2 - We isolated and characterized cDXAs encoding murine cyclm (12 and examined the cell cycle dependent expression of cyclin (1'2 mRNA in marine H cell;-. We investigated the effect of various positive and negative stimuli on the expression of cyclin G2 transcripts in different maturation stage-specific innrine B cell lines. Upon treatment of B cells with the negative growth factor TGF-Jl or growth inhibiting corticostoroids. cyclin (12 m UN A levels are increased 10-11 fold. Although untreated immature B cells (WKHI-2;il and CHlil ) and B cell receptor (BCR} cross-linked or phorbol ester plus ionoinycin stimulated mature B cells (BAL-17 and CH 1'2} proliferate and express low levels of cyclin CI2 mRNA relative to cyclin 1)2. similarly stimulated immature B cells undergo growth arrest and apoptosis concurrent with an approximate ](} fold increase in cyclin G2 transcripts and decrease in cylin 1)2 niRNAs. In contract. BCR stimulated WKHI-231 phosphoinositide signaling mutants re sis-tani to BCR induced growth arrest did not display an increase in cyclin (12 or a decrease in cyclin 1)2 mRNAs. Costirnulation ol BCR challenged immature B cells with LPS or gp39 blocked cyclin G2 rnRXA upregulation and ihe concomitant growth arrest response. Rat cyclui (12 and cyclin I ha.s recently been cloned from a rat brain c.DNA library allowing an examination of its ex pression pattern in neuronal eell lines and tissues undergoing differentiation, growth arrest diid apoptosis.
AB - We isolated and characterized cDXAs encoding murine cyclm (12 and examined the cell cycle dependent expression of cyclin (1'2 mRNA in marine H cell;-. We investigated the effect of various positive and negative stimuli on the expression of cyclin G2 transcripts in different maturation stage-specific innrine B cell lines. Upon treatment of B cells with the negative growth factor TGF-Jl or growth inhibiting corticostoroids. cyclin (12 m UN A levels are increased 10-11 fold. Although untreated immature B cells (WKHI-2;il and CHlil ) and B cell receptor (BCR} cross-linked or phorbol ester plus ionoinycin stimulated mature B cells (BAL-17 and CH 1'2} proliferate and express low levels of cyclin CI2 mRNA relative to cyclin 1)2. similarly stimulated immature B cells undergo growth arrest and apoptosis concurrent with an approximate ](} fold increase in cyclin G2 transcripts and decrease in cylin 1)2 niRNAs. In contract. BCR stimulated WKHI-231 phosphoinositide signaling mutants re sis-tani to BCR induced growth arrest did not display an increase in cyclin (12 or a decrease in cyclin 1)2 mRNAs. Costirnulation ol BCR challenged immature B cells with LPS or gp39 blocked cyclin G2 rnRXA upregulation and ihe concomitant growth arrest response. Rat cyclui (12 and cyclin I ha.s recently been cloned from a rat brain c.DNA library allowing an examination of its ex pression pattern in neuronal eell lines and tissues undergoing differentiation, growth arrest diid apoptosis.
UR - http://www.scopus.com/inward/record.url?scp=33750240445&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750240445&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33750240445
VL - 11
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 9
ER -