Cyclin B1/Cdk1 coordinates mitochondrial respiration for Cell-Cycle G2/M progression

Zhaoqing Wang, Ming Fan, Demet Candas, Tie Qiao Zhang, Lili Qin, Angela Eldridge, Sebastian Wachsmann-Hogiu, Kazi M. Ahmed, Brett A. Chromy, Danupon Nantajit, Nadire Duru, Fuchu He, Min Chen, Toren Finkel, Lee S. Weinstein, Jian-Jian Li

Research output: Contribution to journalArticle

135 Scopus citations

Abstract

A substantial amount of mitochondrial energy is required for cell-cycle progression. The mechanisms underlying the coordination of the mitochondrial respiration with cell-cycle progression, especially the G2/M transition, remain to be elucidated. Here, we show that a fraction of cyclin B1/Cdk1 proteins localizes to the matrix of mitochondria and phosphorylates a cluster of mitochondrial proteins, including the complex I (CI) subunits in the respiratory chain. Cyclin B1/Cdk1-mediated CI phosphorylation enhances CI activity, whereas deficiency of such phosphorylation in each of the relevant CI subunits results in impairment of CI function. Mitochondria-targeted cyclin B1/Cdk1 increases mitochondrial respiration with enhanced oxygen consumption and ATP generation, which provides cells with efficient bioenergy for G2/M transition and shortens overall cell-cycle time. Thus, cyclin B1/Cdk1-mediated phosphorylation of mitochondrial substrates allows cells to sense and respond to increased energy demand for G2/M transition and, subsequently, to upregulate mitochondrial respiration for successful cell-cycle progression.

Original languageEnglish (US)
Pages (from-to)217-232
Number of pages16
JournalDevelopmental Cell
Volume29
Issue number2
DOIs
StatePublished - Apr 28 2014

ASJC Scopus subject areas

  • Developmental Biology

Fingerprint Dive into the research topics of 'Cyclin B1/Cdk1 coordinates mitochondrial respiration for Cell-Cycle G2/M progression'. Together they form a unique fingerprint.

  • Cite this

    Wang, Z., Fan, M., Candas, D., Zhang, T. Q., Qin, L., Eldridge, A., Wachsmann-Hogiu, S., Ahmed, K. M., Chromy, B. A., Nantajit, D., Duru, N., He, F., Chen, M., Finkel, T., Weinstein, L. S., & Li, J-J. (2014). Cyclin B1/Cdk1 coordinates mitochondrial respiration for Cell-Cycle G2/M progression. Developmental Cell, 29(2), 217-232. https://doi.org/10.1016/j.devcel.2014.03.012