Cyclic GMP/cyclic GMP-dependent protein kinase system prevents excitotoxicity in an immortalized oligodendroglial cell line

Akira Yoshioka, Yoko Yamaya, Shinji Saiki, Masumi Kanemoto, Genjiro Hirose, David E Pleasure

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Previously, we have demonstrated that excitotoxicity of oligodendrocyte- like cells (OLC), differentiated from immortalized rat O-2A progenitor cells (CG-4 cells), is prevented by cyclic AMP-elevating agents. We now report that some agents that elevate cyclic GMP prevent OLC excitotoxicity. Kainate- induced injury was prevented by cyclic GMP analogues (8-bromo-cyclic GMP and dibutyryl cyclic GMP), a guanylate cyclase activator [atrial natriuretic peptide (ANP)], and phosphodiesterase inhibitors [3-isobutyl-1-methylxanthine (IBMX), ibudilast, propentofylline, and rolipram]. When both forskolin and 8- bromo-cyclic GMP were added, kainate-induced injury was additively prevented. There was a strong positive correlation between suppression of kainate- induced Ca2+ influx and prevention of injury by these chemicals. The measurement of intracellular cyclic AMP and cyclic GMP by radioimmunoassay demonstrated the following: an increase of cyclic GMP with treatment with 8- bromo-cyclic GMP, dibutyryl cyclic GMP, and ANP; an increase of cyclic AMP with treatment with ibudilast and rolipram; and an increase of both cyclic AMP and cyclic GMP with treatment with IBMX and propentofylline. Kainate- induced Ca2+ influx was decreased by 8-(4-chlorophenylthiol)-guanosine- 3',5'-monophosphate, an activator of cyclic GMP-dependent protein kinase (PKG), or okadaic acid, an inhibitor of protein phosphatases 1 and 2A. RT-PCR and western blotting of OLC demonstrated transcription of PKG II gene and translation of PKG 1β mRNA, but no translation of PKG Iα mRNA. Therefore, we concluded that the cyclic GMP/PKG system prevents OLC excitotoxicity.

Original languageEnglish (US)
Pages (from-to)633-640
Number of pages8
JournalJournal of Neurochemistry
Issue number2
StatePublished - 2000
Externally publishedYes


  • Ca influx
  • Cyclic GMP
  • Cyclic GMP-dependent protein kinase
  • Non-NMDA glutamate receptors
  • Oligodendroglia
  • Protein phosphatases

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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