Cyclic AMP blocks expression of the c-sis gene in tumor cells

G. R. Harsh, W. M. Kavanaugh, N. F. Starksen, L. T. Williams

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Some human tumor cell lines express the c-sis gene, the proto-oncogene of the transforming gene v-sis, and produce platelet-derived growth factor, which may contribute to carcinogenesis by autocrine or paracrine mechanisms. Hence we demonstrate that c-sis expression in some human glioma and osteosarcoma cell lines can be blocked by agents that increase cellular cyclic adenosine monophosphate (cAMP). Forskolin, 8-bromocyclic AMP, cholera toxin, and prostaglandin E1 reduced c-sis mRNA in these cells by up to 90%. c-sis transcription rates were reduced by agents that increase cAMP; the stability of c-sis mRNA was unaffected. The possible therapeutic value of blocking the expression of tumor growth factor genes pharmacologically warrants further study.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalOncogene Research
Volume4
Issue number1
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research

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