Cutting edge: Role of NK cells and surfactant protein D in dendritic cell lymph node homing: Effects of ozone exposure

Moyar Qing Ge, Blerina Kokalari, Cameron H. Flayer, Sarah S. Killingbeck, Imre G. Redai, Alexander W. MacFarlane, Jin W. Hwang, Anisha Kolupoti, Michael D. Kemeny, Kerry S. Campbell, Angela Franciska Haczku

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The roles of NK cells, surfactant protein D (SP-D), and IFN-γ, as well as the effect of ozone (O3) inhalation, were studied on recirculation of pulmonary dendritic cells (DC) to the mediastinal lymph nodes. O3 exposure and lack of SP-D reduced NK cell IFN-γ and lung tissue CCL21 mRNA expression and impaired DC homing to the mediastinal lymph nodes. Notably, addition of recombinant SP-D to naive mononuclear cells stimulated IFN-γ release in vitro. Because NKp46, a glycosylated membrane receptor, was necessary for dosedependent SP-D binding to NK cells in vitro and DC migration in vivo, we speculate that SP-D may constitutively stimulate IFN-γ production by NK cells, possibly via NKp46. This mechanism could then initiate the IFN-γ/IL-12 feedback circuit, a key amplifier of DC lymph node homing. Inhibition of this process during an acute inflammatory response causes DC retention in the peripheral lung tissue and contributes to injury.

Original languageEnglish (US)
Pages (from-to)553-557
Number of pages5
JournalJournal of Immunology
Volume196
Issue number2
DOIs
StatePublished - Jan 15 2016

ASJC Scopus subject areas

  • Immunology

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