Cutting Edge: Rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells

Victor Huang, Anke S. Lonsdorf, Lei Fang, Takashi Kakinuma, Vivian C. Lee, Emily Cha, Hong Zhang, Keisuke Nagao, Marzanna Zaleska, Waldemar L. Olszewski, Samuel T Hwang

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

CC chemokine receptor 10 and its ligand, CCL27, are important components of T cell-mediated cutaneous immunity, but whether they influence lymph node (LN) homing by T cells is unknown. In this study, CCL27 protein was detected in skin-draining LN by Western blotting and ELISA although CCL27 mRNA transcripts were low. CCL27 protein was present at higher levels in skin-draining LN compared with gut-draining LN and spleen. A single topical treatment of mouse skin with the contact sensitizer 2,4-dinitro-1-fluorobenzene (DNFB) resulted in a 13-fold increase in CCL27 protein accumulation in skin-draining LN within 1 h and a 5-fold elevation in CCR10 mRNA (normalized to the T cell marker CD2) within 6 h. DNFB treatment also resulted in rapid depletion of ∼75% of CCL27 from the epidermis. In summary, we describe a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis.

Original languageEnglish (US)
Pages (from-to)6462-6466
Number of pages5
JournalJournal of Immunology
Volume180
Issue number10
StatePublished - 2008
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Cutting Edge: Rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells'. Together they form a unique fingerprint.

Cite this