Cutting Edge: Rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells

Victor Huang, Anke S. Lonsdorf, Lei Fang, Takashi Kakinuma, Vivian C. Lee, Emily Cha, Hong Zhang, Keisuke Nagao, Marzanna Zaleska, Waldemar L. Olszewski, Samuel T Hwang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

CC chemokine receptor 10 and its ligand, CCL27, are important components of T cell-mediated cutaneous immunity, but whether they influence lymph node (LN) homing by T cells is unknown. In this study, CCL27 protein was detected in skin-draining LN by Western blotting and ELISA although CCL27 mRNA transcripts were low. CCL27 protein was present at higher levels in skin-draining LN compared with gut-draining LN and spleen. A single topical treatment of mouse skin with the contact sensitizer 2,4-dinitro-1-fluorobenzene (DNFB) resulted in a 13-fold increase in CCL27 protein accumulation in skin-draining LN within 1 h and a 5-fold elevation in CCR10 mRNA (normalized to the T cell marker CD2) within 6 h. DNFB treatment also resulted in rapid depletion of ∼75% of CCL27 from the epidermis. In summary, we describe a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis.

Original languageEnglish (US)
Pages (from-to)6462-6466
Number of pages5
JournalJournal of Immunology
Volume180
Issue number10
StatePublished - 2008
Externally publishedYes

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Lymph Nodes
T-Lymphocytes
Skin
Fluorobenzenes
Epidermis
CCR10 Receptors
Messenger RNA
Proteins
Cellular Immunity
Spleen
Western Blotting
Enzyme-Linked Immunosorbent Assay
Ligands
Therapeutics

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting Edge : Rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells. / Huang, Victor; Lonsdorf, Anke S.; Fang, Lei; Kakinuma, Takashi; Lee, Vivian C.; Cha, Emily; Zhang, Hong; Nagao, Keisuke; Zaleska, Marzanna; Olszewski, Waldemar L.; Hwang, Samuel T.

In: Journal of Immunology, Vol. 180, No. 10, 2008, p. 6462-6466.

Research output: Contribution to journalArticle

Huang, V, Lonsdorf, AS, Fang, L, Kakinuma, T, Lee, VC, Cha, E, Zhang, H, Nagao, K, Zaleska, M, Olszewski, WL & Hwang, ST 2008, 'Cutting Edge: Rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells', Journal of Immunology, vol. 180, no. 10, pp. 6462-6466.
Huang, Victor ; Lonsdorf, Anke S. ; Fang, Lei ; Kakinuma, Takashi ; Lee, Vivian C. ; Cha, Emily ; Zhang, Hong ; Nagao, Keisuke ; Zaleska, Marzanna ; Olszewski, Waldemar L. ; Hwang, Samuel T. / Cutting Edge : Rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells. In: Journal of Immunology. 2008 ; Vol. 180, No. 10. pp. 6462-6466.
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abstract = "CC chemokine receptor 10 and its ligand, CCL27, are important components of T cell-mediated cutaneous immunity, but whether they influence lymph node (LN) homing by T cells is unknown. In this study, CCL27 protein was detected in skin-draining LN by Western blotting and ELISA although CCL27 mRNA transcripts were low. CCL27 protein was present at higher levels in skin-draining LN compared with gut-draining LN and spleen. A single topical treatment of mouse skin with the contact sensitizer 2,4-dinitro-1-fluorobenzene (DNFB) resulted in a 13-fold increase in CCL27 protein accumulation in skin-draining LN within 1 h and a 5-fold elevation in CCR10 mRNA (normalized to the T cell marker CD2) within 6 h. DNFB treatment also resulted in rapid depletion of ∼75{\%} of CCL27 from the epidermis. In summary, we describe a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis.",
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AU - Huang, Victor

AU - Lonsdorf, Anke S.

AU - Fang, Lei

AU - Kakinuma, Takashi

AU - Lee, Vivian C.

AU - Cha, Emily

AU - Zhang, Hong

AU - Nagao, Keisuke

AU - Zaleska, Marzanna

AU - Olszewski, Waldemar L.

AU - Hwang, Samuel T

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AB - CC chemokine receptor 10 and its ligand, CCL27, are important components of T cell-mediated cutaneous immunity, but whether they influence lymph node (LN) homing by T cells is unknown. In this study, CCL27 protein was detected in skin-draining LN by Western blotting and ELISA although CCL27 mRNA transcripts were low. CCL27 protein was present at higher levels in skin-draining LN compared with gut-draining LN and spleen. A single topical treatment of mouse skin with the contact sensitizer 2,4-dinitro-1-fluorobenzene (DNFB) resulted in a 13-fold increase in CCL27 protein accumulation in skin-draining LN within 1 h and a 5-fold elevation in CCR10 mRNA (normalized to the T cell marker CD2) within 6 h. DNFB treatment also resulted in rapid depletion of ∼75% of CCL27 from the epidermis. In summary, we describe a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis.

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