Cutting edge: CTLA-4Ig inhibits memory B cell responses and promotes allograft survival in sensitized recipients

Jianjun Chen, Qiang Wang, Dengping Yin, Vinh Vu, Roger Sciammas, Anita S. Chong

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Sensitized recipients with pretransplant donor-specific Abs are at higher risk for Ab-mediated rejection than nonsensitized recipients, yet little is known about the properties of memory B cells that are central to the recall alloantibody responses. Using cell enrichment and MHC class I tetramers, C57BL/6 mice sensitized with BALB/c splenocytes were shown to harbor H-2Kd-specific IgG+ memory B cells with a post-germinal center phenotype (CD73+CD273+CD38hiCD138-GL7-). These memory B cells adoptively transferred into naive mice without memory T cells recapitulated class-switched recall alloantibody responses. During recall, memory H-2Kd-specific B cells preferentially differentiated into Ab-secreting cells, whereas in the primary response, H-2Kd-specific B cells differentiated into germinal center cells. Finally, our studies revealed that, despite fundamental differences in alloreactive B cell fates in sensitized versus naive recipients, CTLA-4Ig was unexpectedly effective at constraining B cell responses and heart allograft rejection in sensitized recipients.

Original languageEnglish (US)
Pages (from-to)4069-4073
Number of pages5
JournalJournal of Immunology
Volume195
Issue number9
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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