Abstract
The binding of a T cell to an Ag-laden dendritic cell (DC) is a critical step of the acquired immune response. Herein, we address whether a DC-produced chemokine can induce the arrest of T cells on DC under dynamic flow conditions. Ag-primed T cells and a T cell line were observed to rapidly (∼0.5 s) bind to immobilized DC at low shear stress (0.1-0.2 dynes/cm 2) in a pertussis toxin-sensitive fashion. Quantitatively, Ag-primed T cells displayed 2- to 3-fold enhanced binding to DC compared with unprimed T cells (p < 0.01). In contrast to naive T cells, primed T cell arrest was largely inhibited by pertussis toxin, neutralization of the CC chemokine, macrophage-derived chemokine (CCL22), or by desensitization of the CCL22 receptor, CCR4. Our results demonstrate that DC-derived CCL22 induces rapid binding of activated T cells under dynamic conditions and that Ag-primed and naive T cells fundamentally differ with respect to chemokine -dependent binding to DC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4791-4795 |
| Number of pages | 5 |
| Journal | Journal of Immunology |
| Volume | 167 |
| Issue number | 9 |
| State | Published - Nov 1 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Cutting edge : CCR4 mediates antigen-primed T cell binding to activated dendritic cells. / Wu, M. T.; Fang, H.; Hwang, Samuel T.
In: Journal of Immunology, Vol. 167, No. 9, 01.11.2001, p. 4791-4795.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cutting edge
T2 - CCR4 mediates antigen-primed T cell binding to activated dendritic cells
AU - Wu, M. T.
AU - Fang, H.
AU - Hwang, Samuel T
PY - 2001/11/1
Y1 - 2001/11/1
N2 - The binding of a T cell to an Ag-laden dendritic cell (DC) is a critical step of the acquired immune response. Herein, we address whether a DC-produced chemokine can induce the arrest of T cells on DC under dynamic flow conditions. Ag-primed T cells and a T cell line were observed to rapidly (∼0.5 s) bind to immobilized DC at low shear stress (0.1-0.2 dynes/cm 2) in a pertussis toxin-sensitive fashion. Quantitatively, Ag-primed T cells displayed 2- to 3-fold enhanced binding to DC compared with unprimed T cells (p < 0.01). In contrast to naive T cells, primed T cell arrest was largely inhibited by pertussis toxin, neutralization of the CC chemokine, macrophage-derived chemokine (CCL22), or by desensitization of the CCL22 receptor, CCR4. Our results demonstrate that DC-derived CCL22 induces rapid binding of activated T cells under dynamic conditions and that Ag-primed and naive T cells fundamentally differ with respect to chemokine -dependent binding to DC.
AB - The binding of a T cell to an Ag-laden dendritic cell (DC) is a critical step of the acquired immune response. Herein, we address whether a DC-produced chemokine can induce the arrest of T cells on DC under dynamic flow conditions. Ag-primed T cells and a T cell line were observed to rapidly (∼0.5 s) bind to immobilized DC at low shear stress (0.1-0.2 dynes/cm 2) in a pertussis toxin-sensitive fashion. Quantitatively, Ag-primed T cells displayed 2- to 3-fold enhanced binding to DC compared with unprimed T cells (p < 0.01). In contrast to naive T cells, primed T cell arrest was largely inhibited by pertussis toxin, neutralization of the CC chemokine, macrophage-derived chemokine (CCL22), or by desensitization of the CCL22 receptor, CCR4. Our results demonstrate that DC-derived CCL22 induces rapid binding of activated T cells under dynamic conditions and that Ag-primed and naive T cells fundamentally differ with respect to chemokine -dependent binding to DC.
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M3 - Article
C2 - 11673480
AN - SCOPUS:0035500756
VL - 167
SP - 4791
EP - 4795
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 9
ER -