Current peptidomics: Applications, purification, identification, quantification, and functional analysis

David C. Dallas, Andres Guerrero, Evan A. Parker, Randall C. Robinson, Junai Gan, J. Bruce German, Daniela Barile, Carlito B Lebrilla

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Peptidomics is an emerging field branching from proteomics that targets endogenously produced protein fragments. Endogenous peptides are often functional within the body-and can be both beneficial and detrimental. This review covers the use of peptidomics in understanding digestion, and identifying functional peptides and biomarkers. Various techniques for peptide and glycopeptide extraction, both at analytical and preparative scales, and available options for peptide detection with MS are discussed. Current algorithms for peptide sequence determination, and both analytical and computational techniques for quantification are compared. Techniques for statistical analysis, sequence mapping, enzyme prediction, and peptide function, and structure prediction are explored.

Original languageEnglish (US)
Pages (from-to)1026-1038
Number of pages13
JournalProteomics
Volume15
Issue number5-6
DOIs
StatePublished - Mar 1 2015

Keywords

  • Functional prediction
  • MS
  • Peptidomics
  • Quantitation
  • Structure prediction
  • Systems biology

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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    Dallas, D. C., Guerrero, A., Parker, E. A., Robinson, R. C., Gan, J., German, J. B., Barile, D., & Lebrilla, C. B. (2015). Current peptidomics: Applications, purification, identification, quantification, and functional analysis. Proteomics, 15(5-6), 1026-1038. https://doi.org/10.1002/pmic.201400310