Current methods for the analysis of human milk oligosaccharides (HMOs) and their novel applications

L. R. Ruhaak, Carlito B Lebrilla

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

The microflora in the gastrointestinal tract of an infant plays an important role in the development of the immune system. Human milk oligosaccharides (HMOs)have become the subject of considerable interest because they strongly influence the composition of the gut microflora. The structures of the HMOs are usually very complex and novel techniques such as porous graphitic carbon-liquid chromatography-mass spectrometry (PGC-LC-MS) now facilitate separation and identification of most of the isomers. In contrast, matrix assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis may now generate fast profiles, but does not allow isomer separation. Application of these novel techniques allows more accurate studies of Lewis blood group determinants, phylogenetic differences in milk oligosaccharide composition and bacterial HMO consumption. Such studies will greatly enhance knowledge of the biological functions of HMOs.

Original languageEnglish (US)
Title of host publicationMetabolomics in Food and Nutrition
PublisherElsevier Inc.
Pages124-147
Number of pages24
ISBN (Print)9781845695125
DOIs
StatePublished - Oct 2013

Fingerprint

Human Milk
breast milk
Oligosaccharides
oligosaccharides
Isomers
isomers
Mass spectrometry
Mass Spectrometry
methodology
matrix-assisted laser desorption-ionization mass spectrometry
Immune system
blood groups
Liquid chromatography
Blood Group Antigens
Chemical analysis
intestinal microorganisms
Liquid Chromatography
liquid chromatography
Ionization
gastrointestinal system

Keywords

  • Analysis
  • Bacterial consumption
  • High performance liquid chromatography
  • Lewis blood group
  • Mass spectrometry
  • Milk oligosaccharides
  • Secretor status

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ruhaak, L. R., & Lebrilla, C. B. (2013). Current methods for the analysis of human milk oligosaccharides (HMOs) and their novel applications. In Metabolomics in Food and Nutrition (pp. 124-147). Elsevier Inc.. https://doi.org/10.1533/9780857098818.2.124

Current methods for the analysis of human milk oligosaccharides (HMOs) and their novel applications. / Ruhaak, L. R.; Lebrilla, Carlito B.

Metabolomics in Food and Nutrition. Elsevier Inc., 2013. p. 124-147.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ruhaak, LR & Lebrilla, CB 2013, Current methods for the analysis of human milk oligosaccharides (HMOs) and their novel applications. in Metabolomics in Food and Nutrition. Elsevier Inc., pp. 124-147. https://doi.org/10.1533/9780857098818.2.124
Ruhaak LR, Lebrilla CB. Current methods for the analysis of human milk oligosaccharides (HMOs) and their novel applications. In Metabolomics in Food and Nutrition. Elsevier Inc. 2013. p. 124-147 https://doi.org/10.1533/9780857098818.2.124
Ruhaak, L. R. ; Lebrilla, Carlito B. / Current methods for the analysis of human milk oligosaccharides (HMOs) and their novel applications. Metabolomics in Food and Nutrition. Elsevier Inc., 2013. pp. 124-147
@inbook{abe635f94d874d0cb2fbf7d923d07ef5,
title = "Current methods for the analysis of human milk oligosaccharides (HMOs) and their novel applications",
abstract = "The microflora in the gastrointestinal tract of an infant plays an important role in the development of the immune system. Human milk oligosaccharides (HMOs)have become the subject of considerable interest because they strongly influence the composition of the gut microflora. The structures of the HMOs are usually very complex and novel techniques such as porous graphitic carbon-liquid chromatography-mass spectrometry (PGC-LC-MS) now facilitate separation and identification of most of the isomers. In contrast, matrix assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis may now generate fast profiles, but does not allow isomer separation. Application of these novel techniques allows more accurate studies of Lewis blood group determinants, phylogenetic differences in milk oligosaccharide composition and bacterial HMO consumption. Such studies will greatly enhance knowledge of the biological functions of HMOs.",
keywords = "Analysis, Bacterial consumption, High performance liquid chromatography, Lewis blood group, Mass spectrometry, Milk oligosaccharides, Secretor status",
author = "Ruhaak, {L. R.} and Lebrilla, {Carlito B}",
year = "2013",
month = "10",
doi = "10.1533/9780857098818.2.124",
language = "English (US)",
isbn = "9781845695125",
pages = "124--147",
booktitle = "Metabolomics in Food and Nutrition",
publisher = "Elsevier Inc.",

}

TY - CHAP

T1 - Current methods for the analysis of human milk oligosaccharides (HMOs) and their novel applications

AU - Ruhaak, L. R.

AU - Lebrilla, Carlito B

PY - 2013/10

Y1 - 2013/10

N2 - The microflora in the gastrointestinal tract of an infant plays an important role in the development of the immune system. Human milk oligosaccharides (HMOs)have become the subject of considerable interest because they strongly influence the composition of the gut microflora. The structures of the HMOs are usually very complex and novel techniques such as porous graphitic carbon-liquid chromatography-mass spectrometry (PGC-LC-MS) now facilitate separation and identification of most of the isomers. In contrast, matrix assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis may now generate fast profiles, but does not allow isomer separation. Application of these novel techniques allows more accurate studies of Lewis blood group determinants, phylogenetic differences in milk oligosaccharide composition and bacterial HMO consumption. Such studies will greatly enhance knowledge of the biological functions of HMOs.

AB - The microflora in the gastrointestinal tract of an infant plays an important role in the development of the immune system. Human milk oligosaccharides (HMOs)have become the subject of considerable interest because they strongly influence the composition of the gut microflora. The structures of the HMOs are usually very complex and novel techniques such as porous graphitic carbon-liquid chromatography-mass spectrometry (PGC-LC-MS) now facilitate separation and identification of most of the isomers. In contrast, matrix assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) analysis may now generate fast profiles, but does not allow isomer separation. Application of these novel techniques allows more accurate studies of Lewis blood group determinants, phylogenetic differences in milk oligosaccharide composition and bacterial HMO consumption. Such studies will greatly enhance knowledge of the biological functions of HMOs.

KW - Analysis

KW - Bacterial consumption

KW - High performance liquid chromatography

KW - Lewis blood group

KW - Mass spectrometry

KW - Milk oligosaccharides

KW - Secretor status

UR - http://www.scopus.com/inward/record.url?scp=84904232059&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904232059&partnerID=8YFLogxK

U2 - 10.1533/9780857098818.2.124

DO - 10.1533/9780857098818.2.124

M3 - Chapter

AN - SCOPUS:84904232059

SN - 9781845695125

SP - 124

EP - 147

BT - Metabolomics in Food and Nutrition

PB - Elsevier Inc.

ER -