Abstract
Dityrosine is a fluorescent molecule formed as a result of normal posttranslational processing. In many structural proteins, dityrosine confera resistance to proteolysis and physicochemical trauma as a stabilizing crosslink. Dityrosine has also been found in oxidative/nitrative stress under a variety of conditions and biological systems. In this regard, it has been used as an important biomarker for oxidatively modified proteins during UV and γ-irradiation, aging, and exposure to oxygen free radicals, nitrogen dioxide, peroxynitrite, and lipid hydroperoxides. Renewed interest in dityrosine and other tyrosine oxidation products as clinical indicators of oxidative modification has driven the development of important techniques for the specific analysis and quantification of these molecules. The presence of elevated levels of dityrosine in mammalian tissue and urine samples has been measured by chromatographic separation followed by mass spectrometry GC-MS and HPLC-MS/MS. Increases in dityrosine levels have been associated with pathologies such as eye cataracts, atherosclerosis, acute inflammation, and Alzheimer's disease. The continued development of, and increased accessibility to, improved mass spectrometric instrumentation will expand the capability, feasibility, and sensitivity with which specific biomarkers like dityrosine can be measured.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 108-120 |
| Number of pages | 13 |
| Journal | Mass Spectrometry Reviews |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2007 |
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Keywords
- Biomarker
- Dityrosine
- Free radicals
- Mass spectrometry
- Oxidative stress
- Posttranslational modification
- Protein damage
ASJC Scopus subject areas
- Spectroscopy
Cite this
Current analytical methods for the detection of dityrosine, a biomarker of oxidative stress, in biological samples. / DiMarco, Theresa; Giulivi, Cecilia R.
In: Mass Spectrometry Reviews, Vol. 26, No. 1, 01.2007, p. 108-120.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Current analytical methods for the detection of dityrosine, a biomarker of oxidative stress, in biological samples
AU - DiMarco, Theresa
AU - Giulivi, Cecilia R
PY - 2007/1
Y1 - 2007/1
N2 - Dityrosine is a fluorescent molecule formed as a result of normal posttranslational processing. In many structural proteins, dityrosine confera resistance to proteolysis and physicochemical trauma as a stabilizing crosslink. Dityrosine has also been found in oxidative/nitrative stress under a variety of conditions and biological systems. In this regard, it has been used as an important biomarker for oxidatively modified proteins during UV and γ-irradiation, aging, and exposure to oxygen free radicals, nitrogen dioxide, peroxynitrite, and lipid hydroperoxides. Renewed interest in dityrosine and other tyrosine oxidation products as clinical indicators of oxidative modification has driven the development of important techniques for the specific analysis and quantification of these molecules. The presence of elevated levels of dityrosine in mammalian tissue and urine samples has been measured by chromatographic separation followed by mass spectrometry GC-MS and HPLC-MS/MS. Increases in dityrosine levels have been associated with pathologies such as eye cataracts, atherosclerosis, acute inflammation, and Alzheimer's disease. The continued development of, and increased accessibility to, improved mass spectrometric instrumentation will expand the capability, feasibility, and sensitivity with which specific biomarkers like dityrosine can be measured.
AB - Dityrosine is a fluorescent molecule formed as a result of normal posttranslational processing. In many structural proteins, dityrosine confera resistance to proteolysis and physicochemical trauma as a stabilizing crosslink. Dityrosine has also been found in oxidative/nitrative stress under a variety of conditions and biological systems. In this regard, it has been used as an important biomarker for oxidatively modified proteins during UV and γ-irradiation, aging, and exposure to oxygen free radicals, nitrogen dioxide, peroxynitrite, and lipid hydroperoxides. Renewed interest in dityrosine and other tyrosine oxidation products as clinical indicators of oxidative modification has driven the development of important techniques for the specific analysis and quantification of these molecules. The presence of elevated levels of dityrosine in mammalian tissue and urine samples has been measured by chromatographic separation followed by mass spectrometry GC-MS and HPLC-MS/MS. Increases in dityrosine levels have been associated with pathologies such as eye cataracts, atherosclerosis, acute inflammation, and Alzheimer's disease. The continued development of, and increased accessibility to, improved mass spectrometric instrumentation will expand the capability, feasibility, and sensitivity with which specific biomarkers like dityrosine can be measured.
KW - Biomarker
KW - Dityrosine
KW - Free radicals
KW - Mass spectrometry
KW - Oxidative stress
KW - Posttranslational modification
KW - Protein damage
UR - http://www.scopus.com/inward/record.url?scp=33845967456&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845967456&partnerID=8YFLogxK
U2 - 10.1002/mas.20109
DO - 10.1002/mas.20109
M3 - Article
C2 - 17019703
AN - SCOPUS:33845967456
VL - 26
SP - 108
EP - 120
JO - Mass Spectrometry Reviews
JF - Mass Spectrometry Reviews
SN - 0277-7037
IS - 1
ER -