Cultures of bovine tracheal epithelium with differentiated ultrastructure and ion transport

M. Kondo, W. E. Finkbeiner, Jonathan Widdicombe

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Tracheal epithelial cells were grown on Nuclepore filters coated with human placental collagen. When grown immersed in medium containing fetal bovine serum, cells displayed an undifferentiated ultrastructure (no cilia and a cell height of ~ 10 μm). Short-circuit current (I(sc)) was approximately 1/10 that of the native epithelium. By contrast, when grown in hormonally defined, serum-free medium with an air interface, cells showed I(sc) equal to or greater than the original tissue, possessed cilia, and had a cell height of ~ 50 μm. Responses in I(sc) to mediators were similar to those of the original tissue but differed from those of dog or human tracheal epithelium. Given the ready availability and low cost of the native tissues, bovine tracheal cultures grown in serum-free medium with an air interface should prove useful in studies of airway epithelial physiology.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume29 A
Issue number1
StatePublished - 1993
Externally publishedYes

Keywords

  • Chloride transport
  • Ciliated epithelium
  • Hormonally defined medium
  • Ussing chambers

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

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