Abstract
Tracheal epithelial cells were grown on Nuclepore filters coated with human placental collagen. When grown immersed in medium containing fetal bovine serum, cells displayed an undifferentiated ultrastructure (no cilia and a cell height of ~ 10 μm). Short-circuit current (I(sc)) was approximately 1/10 that of the native epithelium. By contrast, when grown in hormonally defined, serum-free medium with an air interface, cells showed I(sc) equal to or greater than the original tissue, possessed cilia, and had a cell height of ~ 50 μm. Responses in I(sc) to mediators were similar to those of the original tissue but differed from those of dog or human tracheal epithelium. Given the ready availability and low cost of the native tissues, bovine tracheal cultures grown in serum-free medium with an air interface should prove useful in studies of airway epithelial physiology.
Original language | English (US) |
---|---|
Pages (from-to) | 19-24 |
Number of pages | 6 |
Journal | In Vitro Cellular and Developmental Biology - Animal |
Volume | 29 A |
Issue number | 1 |
State | Published - 1993 |
Externally published | Yes |
Keywords
- Chloride transport
- Ciliated epithelium
- Hormonally defined medium
- Ussing chambers
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Developmental Biology