Cultured rat Schwann cells express low affinity receptors for nerve growth factor

Takeshi Yasuda, Gen Sobue, Kenji Mokuno, Barbara Kreider, David E Pleasure

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Schwann cell cultures prepared from postnatal Sprague-Dawley rat sciatic nerves were used to demonstrate the presence of specific receptors for the β-subunit of nerve growth factor (NGF) on rat Schwann cells. Indirect immunofluorescence microscopy with a monoclonal antineuronal NGF receptor (NGFR) antibody indicated that NGFR antigen was expressed on the surface of Schwann cells but not of endoneurial fibroblasts. Studies with 125I-NGF confirmed this distribution of NGFR in the cultures and showed that the Schwann cell NGFR had a single NGF binding affinity (Kd of 1.8 × 10-9 M). 125I-NGF binding by the cultured Schwann cells increased with time in vitro, reaching a plateau level on the 4th day, but decreased with increasing age, reaching 40% of the neonatal value in Schwann cells isolated from 12-day-old rats. Treatment of the cultures with NGF did not alter Schwann cell phenotype, survival or proliferation.

Original languageEnglish (US)
Pages (from-to)113-119
Number of pages7
JournalBrain Research
Volume436
Issue number1
DOIs
StatePublished - Dec 8 1987
Externally publishedYes

Keywords

  • Nerve growth factor (NGF)
  • Nerve growth factor receptor
  • Schwann cell

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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