Cultured rat Schwann cells express low affinity receptors for nerve growth factor

Takeshi Yasuda, Gen Sobue, Kenji Mokuno, Barbara Kreider, David E Pleasure

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Schwann cell cultures prepared from postnatal Sprague-Dawley rat sciatic nerves were used to demonstrate the presence of specific receptors for the β-subunit of nerve growth factor (NGF) on rat Schwann cells. Indirect immunofluorescence microscopy with a monoclonal antineuronal NGF receptor (NGFR) antibody indicated that NGFR antigen was expressed on the surface of Schwann cells but not of endoneurial fibroblasts. Studies with 125I-NGF confirmed this distribution of NGFR in the cultures and showed that the Schwann cell NGFR had a single NGF binding affinity (Kd of 1.8 × 10-9 M). 125I-NGF binding by the cultured Schwann cells increased with time in vitro, reaching a plateau level on the 4th day, but decreased with increasing age, reaching 40% of the neonatal value in Schwann cells isolated from 12-day-old rats. Treatment of the cultures with NGF did not alter Schwann cell phenotype, survival or proliferation.

Original languageEnglish (US)
Pages (from-to)113-119
Number of pages7
JournalBrain Research
Volume436
Issue number1
DOIs
StatePublished - Dec 8 1987
Externally publishedYes

Fingerprint

Nerve Growth Factor Receptor
Schwann Cells
Nerve Growth Factor
Nerve Growth Factor Receptors
Sciatic Nerve
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Sprague Dawley Rats
Cultured Cells
Cell Survival
Cell Culture Techniques
Fibroblasts
Cell Proliferation
Phenotype
Antigens
Antibodies

Keywords

  • Nerve growth factor (NGF)
  • Nerve growth factor receptor
  • Schwann cell

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Cultured rat Schwann cells express low affinity receptors for nerve growth factor. / Yasuda, Takeshi; Sobue, Gen; Mokuno, Kenji; Kreider, Barbara; Pleasure, David E.

In: Brain Research, Vol. 436, No. 1, 08.12.1987, p. 113-119.

Research output: Contribution to journalArticle

Yasuda, Takeshi ; Sobue, Gen ; Mokuno, Kenji ; Kreider, Barbara ; Pleasure, David E. / Cultured rat Schwann cells express low affinity receptors for nerve growth factor. In: Brain Research. 1987 ; Vol. 436, No. 1. pp. 113-119.
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