Abstract
This chapter presents a model for neuronal function, differentiation, and survival of cultured pheochromocytoma PC12 cell lines. The optimal final dilution of the collagen should be determined empirically by testing various concentrations for their capacities to foster cell attachment and nerve growth factor (NGF)-promoted neurite outgrowth. Spread the collagen evenly over the surface of the culture dish with the use of an L-shaped glass rod. Dry collagen by leaving the plates uncovered for 1-2 h in a tissue culture hood. One needs to store collagen-coated dishes at room temperature and use within one week after preparation. Dislodge the cells from the surface of the dish by repeated and forceful discharge of the culture medium directly onto the cells with a disposable glass Pasteur pipette. Forceful trituration of the cell suspension within the Pasteur pipette also decreases cell clumping. To determine the numbers of PC12 cells suspended in culture or other medium, pellet the cells by centrifugation, aspirate to remove the medium, and resuspend the cells in a known volume of the working nuclei-counting solution.
| Original language | English (US) |
|---|---|
| Title of host publication | Cell Biology, Four-Volume Set |
| Publisher | Elsevier Inc. |
| Pages | 171-176 |
| Number of pages | 6 |
| Volume | 1 |
| ISBN (Print) | 9780121647308 |
| DOIs | |
| State | Published - 2006 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Cultured PC12 cells : A model for neuronal function, differentiation, and survival. / Teng, Kenneth K.; Angelastro, James M; Cunningham, Matthew E.; Greene, Lloyd A.
Cell Biology, Four-Volume Set. Vol. 1 Elsevier Inc., 2006. p. 171-176.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Cultured PC12 cells
T2 - A model for neuronal function, differentiation, and survival
AU - Teng, Kenneth K.
AU - Angelastro, James M
AU - Cunningham, Matthew E.
AU - Greene, Lloyd A.
PY - 2006
Y1 - 2006
N2 - This chapter presents a model for neuronal function, differentiation, and survival of cultured pheochromocytoma PC12 cell lines. The optimal final dilution of the collagen should be determined empirically by testing various concentrations for their capacities to foster cell attachment and nerve growth factor (NGF)-promoted neurite outgrowth. Spread the collagen evenly over the surface of the culture dish with the use of an L-shaped glass rod. Dry collagen by leaving the plates uncovered for 1-2 h in a tissue culture hood. One needs to store collagen-coated dishes at room temperature and use within one week after preparation. Dislodge the cells from the surface of the dish by repeated and forceful discharge of the culture medium directly onto the cells with a disposable glass Pasteur pipette. Forceful trituration of the cell suspension within the Pasteur pipette also decreases cell clumping. To determine the numbers of PC12 cells suspended in culture or other medium, pellet the cells by centrifugation, aspirate to remove the medium, and resuspend the cells in a known volume of the working nuclei-counting solution.
AB - This chapter presents a model for neuronal function, differentiation, and survival of cultured pheochromocytoma PC12 cell lines. The optimal final dilution of the collagen should be determined empirically by testing various concentrations for their capacities to foster cell attachment and nerve growth factor (NGF)-promoted neurite outgrowth. Spread the collagen evenly over the surface of the culture dish with the use of an L-shaped glass rod. Dry collagen by leaving the plates uncovered for 1-2 h in a tissue culture hood. One needs to store collagen-coated dishes at room temperature and use within one week after preparation. Dislodge the cells from the surface of the dish by repeated and forceful discharge of the culture medium directly onto the cells with a disposable glass Pasteur pipette. Forceful trituration of the cell suspension within the Pasteur pipette also decreases cell clumping. To determine the numbers of PC12 cells suspended in culture or other medium, pellet the cells by centrifugation, aspirate to remove the medium, and resuspend the cells in a known volume of the working nuclei-counting solution.
UR - http://www.scopus.com/inward/record.url?scp=84884889229&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884889229&partnerID=8YFLogxK
U2 - 10.1016/B978-012164730-8/50022-8
DO - 10.1016/B978-012164730-8/50022-8
M3 - Chapter
SN - 9780121647308
VL - 1
SP - 171
EP - 176
BT - Cell Biology, Four-Volume Set
PB - Elsevier Inc.
ER -