Abstract
In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 285-292 |
| Number of pages | 8 |
| Journal | Brain Research |
| Volume | 289 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Dec 19 1983 |
| Externally published | Yes |
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Keywords
- Ia
- oligodendrocyte
- Schwann cells
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface. / Lisak, Robert P.; Hirayama, Mikio; Kuchmy, Diana; Rosenzweig, Anthony; Kim, Seung U.; Pleasure, David E; Silberberg, Donald H.
In: Brain Research, Vol. 289, No. 1-2, 19.12.1983, p. 285-292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface
AU - Lisak, Robert P.
AU - Hirayama, Mikio
AU - Kuchmy, Diana
AU - Rosenzweig, Anthony
AU - Kim, Seung U.
AU - Pleasure, David E
AU - Silberberg, Donald H.
PY - 1983/12/19
Y1 - 1983/12/19
N2 - In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.
AB - In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.
KW - Ia
KW - oligodendrocyte
KW - Schwann cells
UR - http://www.scopus.com/inward/record.url?scp=0021091485&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021091485&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(83)90029-X
DO - 10.1016/0006-8993(83)90029-X
M3 - Article
C2 - 6362776
AN - SCOPUS:0021091485
VL - 289
SP - 285
EP - 292
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -