Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface

Robert P. Lisak; Mikio Hirayama; Diana Kuchmy; Anthony Rosenzweig; Seung U. Kim; David E Pleasure; Donald H. Silberberg

doi:10.1016/0006-8993(83)90029-X

Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface

Robert P. Lisak, Mikio Hirayama, Diana Kuchmy, Anthony Rosenzweig, Seung U. Kim, David E Pleasure, Donald H. Silberberg

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.

Original language	English (US)
Pages (from-to)	285-292
Number of pages	8
Journal	Brain Research
Volume	289
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(83)90029-X
State	Published - Dec 19 1983
Externally published	Yes

Keywords

Ia
oligodendrocyte
Schwann cells

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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10.1016/0006-8993(83)90029-X

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Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface. / Lisak, Robert P.; Hirayama, Mikio; Kuchmy, Diana; Rosenzweig, Anthony; Kim, Seung U.; Pleasure, David E; Silberberg, Donald H.

In: Brain Research, Vol. 289, No. 1-2, 19.12.1983, p. 285-292.

Research output: Contribution to journal › Article › peer-review

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abstract = "In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.",

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AU - Rosenzweig, Anthony

AU - Kim, Seung U.

AU - Pleasure, David E

AU - Silberberg, Donald H.

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N2 - In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.

AB - In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.

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Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface

Abstract

Keywords

ASJC Scopus subject areas

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