Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface

Robert P. Lisak, Mikio Hirayama, Diana Kuchmy, Anthony Rosenzweig, Seung U. Kim, David E Pleasure, Donald H. Silberberg

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalBrain Research
Volume289
Issue number1-2
DOIs
StatePublished - Dec 19 1983
Externally publishedYes

Fingerprint

Schwann Cells
Histocompatibility Antigens Class II
Oligodendroglia
Rabbits
Genes
Astrocytes
Fibroblasts
Monoclonal Antibodies
Fc Receptors
Glial Fibrillary Acidic Protein
Latex
Microglia
Surface Antigens
Indirect Fluorescent Antibody Technique
Microspheres
Fibronectins
Radioimmunoassay
Macrophages
galactocerebroside

Keywords

  • Ia
  • oligodendrocyte
  • Schwann cells

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface. / Lisak, Robert P.; Hirayama, Mikio; Kuchmy, Diana; Rosenzweig, Anthony; Kim, Seung U.; Pleasure, David E; Silberberg, Donald H.

In: Brain Research, Vol. 289, No. 1-2, 19.12.1983, p. 285-292.

Research output: Contribution to journalArticle

Lisak, Robert P. ; Hirayama, Mikio ; Kuchmy, Diana ; Rosenzweig, Anthony ; Kim, Seung U. ; Pleasure, David E ; Silberberg, Donald H. / Cultured human and rat oligodendrocytes and rat schwann cells do not have immune response gene associated antigen (Ia) on their surface. In: Brain Research. 1983 ; Vol. 289, No. 1-2. pp. 285-292.
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AB - In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibroblasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.

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