Culture of purified rat astrocytes in serum-free medium supplemented with mitogen

Seung U. Kim, Janet Stern, Myong W. Kim, David E Pleasure

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

We have obtained a highly purified astrocyte population in cultures originating from neonatal (2-5 days) rat cerebrum by use of the selection process provided by a serum-free chemically defined medium (DM). The addition of a glial growth factor isolated from bovine pituitary glands to DM induced in these astrocyte cultures both a stimulation of astrocytic proliferation and a morphological transformation of the astrocytes from flat fibroblastic form to multipolar stellate form.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalBrain Research
Volume274
Issue number1
DOIs
StatePublished - Sep 5 1983
Externally publishedYes

Keywords

  • astrocyte
  • cell culture
  • mitogen supplement

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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