Culture, isolation, and labeling of Anaplasma phagocytophilum for subsequent infection of human neutrophils

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Anaplasma phagocytophilum is the etiologic agent of granulocytic anaplasmosis, a tick-borne, zoonotic, emerging infectious disease. A. phagocytophilum is an obligate intracellular pathogen that primarily resides within membrane-bound, cytoplasmic vacuoles of host neutrophils. Closely related to Ehrlichial and Rickettsial organisms, A. phagocytophilum is a small, fragile, Gram-negative bacterium that presents unique challenges for culture, isolation, enumeration, and labeling. This chapter delineates pathogen-specific considerations for culture and labeling of this organism for subsequent use in assays to examine mechanisms of host cell-pathogen interactions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages159-171
Number of pages13
Volume431
StatePublished - Aug 31 2007

Publication series

NameMethods in Molecular Biology
Volume431
ISSN (Print)10643745

Keywords

  • Anaplasma phagocytophilum
  • Cell culture
  • Fluorescent labeling
  • Granulocytic anaplasmosis
  • Neutrophils

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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