Culture and initial characterization of the secretory response of neoplastic cat mast cells.

Frederick C Mohr, S. K. Dunston

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Mast cells isolated from feline splenic mastocytomas were cultured to study their structural and functional properties. Isolated cells from various cats were grown as monolayer cultures for a mean of 56 days (range, 30 to 76 days). Cat mast cells released allergic mediators in response to compound 48/80, anti-cat serum antibodies, and concanavalin A. On the basis of the finding that secretion from cat mast cells was stimulated by anti-cat serum antibodies and concanavalin A, these cells contain surface-bound immunoglobulins. The presence of mast cell-sensitizing antibodies has been suspected in cats, but never before directly demonstrated. Cultured cat mast cells have cytochemical and functional characteristics common to connective tissue-type mast cells and provide one of the few non-rodent models of cultured cells for the study of this type of mast cell.

Original languageEnglish (US)
Pages (from-to)820-828
Number of pages9
JournalAmerican Journal of Veterinary Research
Volume53
Issue number5
StatePublished - May 1992

ASJC Scopus subject areas

  • veterinary(all)

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