Culture and co-culture of DU145 prostate carcinoma, osteoblasts and HT-29 colon carcinoma cells on a fabricated type III collagen matrix

C. L. Macias, K. Williams, B. Baccetti, T. Renieri, A Hari Reddi, A. Asseffa, V. Hollis, F. Grissom, W. Anderson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

DU145 prostate carcinoma cells cultured on type III collagen possessed a highly migratory potential which was twice as much as HT-29 colon carcinoma cells. Prior to attachment to collagen, DU145 cells were highly reactive for fibronectin and after attachment clear zones between cells and collagen suggested protease activity. HT-29 cells attached to type III collagen forming dome-like polyps, however, right and/or gap junctions were not observed. hFob osteoblasts were co-cultured with DU145 to establish a prostate cancer-collagen matrix barrier-bone cell metastasis model. Osteoblasts maintained their differentiated osceoblastic characteristics on one side of the collagen barrier, demonstrating high alkaline phosphatase, osteocalcin and insulin growth factor (IGF) activities. hFob cell growth was prominent adjacent to demineralized bone matrix particles (BMPs) embedded in type III collagen. The collagen matrix was deteriorated on the DU145 side of the collagen barrier. The DU145-collagen III-hFob model will allow an evaluation of the influence of the matrix on prostate cancer-bone cell interaction and regulation by growth factors.

Original languageEnglish (US)
Pages (from-to)241-253
Number of pages13
JournalJournal of Submicroscopic Cytology and Pathology
Volume34
Issue number3
StatePublished - Jul 2002

Fingerprint

Collagen Type III
Coculture Techniques
Osteoblasts
Prostate
Colon
Collagen
Carcinoma
Bone Matrix
Prostatic Neoplasms
Intercellular Signaling Peptides and Proteins
Bone Neoplasms
HT29 Cells
Gap Junctions
Osteocalcin
Polyps
Fibronectins
Cell Communication
Alkaline Phosphatase
Cultured Cells
Peptide Hydrolases

Keywords

  • DU 145
  • HT29
  • Type III collagen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Culture and co-culture of DU145 prostate carcinoma, osteoblasts and HT-29 colon carcinoma cells on a fabricated type III collagen matrix. / Macias, C. L.; Williams, K.; Baccetti, B.; Renieri, T.; Reddi, A Hari; Asseffa, A.; Hollis, V.; Grissom, F.; Anderson, W.

In: Journal of Submicroscopic Cytology and Pathology, Vol. 34, No. 3, 07.2002, p. 241-253.

Research output: Contribution to journalArticle

Macias, CL, Williams, K, Baccetti, B, Renieri, T, Reddi, AH, Asseffa, A, Hollis, V, Grissom, F & Anderson, W 2002, 'Culture and co-culture of DU145 prostate carcinoma, osteoblasts and HT-29 colon carcinoma cells on a fabricated type III collagen matrix', Journal of Submicroscopic Cytology and Pathology, vol. 34, no. 3, pp. 241-253.
Macias, C. L. ; Williams, K. ; Baccetti, B. ; Renieri, T. ; Reddi, A Hari ; Asseffa, A. ; Hollis, V. ; Grissom, F. ; Anderson, W. / Culture and co-culture of DU145 prostate carcinoma, osteoblasts and HT-29 colon carcinoma cells on a fabricated type III collagen matrix. In: Journal of Submicroscopic Cytology and Pathology. 2002 ; Vol. 34, No. 3. pp. 241-253.
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AU - Williams, K.

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AU - Renieri, T.

AU - Reddi, A Hari

AU - Asseffa, A.

AU - Hollis, V.

AU - Grissom, F.

AU - Anderson, W.

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AB - DU145 prostate carcinoma cells cultured on type III collagen possessed a highly migratory potential which was twice as much as HT-29 colon carcinoma cells. Prior to attachment to collagen, DU145 cells were highly reactive for fibronectin and after attachment clear zones between cells and collagen suggested protease activity. HT-29 cells attached to type III collagen forming dome-like polyps, however, right and/or gap junctions were not observed. hFob osteoblasts were co-cultured with DU145 to establish a prostate cancer-collagen matrix barrier-bone cell metastasis model. Osteoblasts maintained their differentiated osceoblastic characteristics on one side of the collagen barrier, demonstrating high alkaline phosphatase, osteocalcin and insulin growth factor (IGF) activities. hFob cell growth was prominent adjacent to demineralized bone matrix particles (BMPs) embedded in type III collagen. The collagen matrix was deteriorated on the DU145 side of the collagen barrier. The DU145-collagen III-hFob model will allow an evaluation of the influence of the matrix on prostate cancer-bone cell interaction and regulation by growth factors.

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