Abstract
DU145 prostate carcinoma cells cultured on type III collagen possessed a highly migratory potential which was twice as much as HT-29 colon carcinoma cells. Prior to attachment to collagen, DU145 cells were highly reactive for fibronectin and after attachment clear zones between cells and collagen suggested protease activity. HT-29 cells attached to type III collagen forming dome-like polyps, however, right and/or gap junctions were not observed. hFob osteoblasts were co-cultured with DU145 to establish a prostate cancer-collagen matrix barrier-bone cell metastasis model. Osteoblasts maintained their differentiated osceoblastic characteristics on one side of the collagen barrier, demonstrating high alkaline phosphatase, osteocalcin and insulin growth factor (IGF) activities. hFob cell growth was prominent adjacent to demineralized bone matrix particles (BMPs) embedded in type III collagen. The collagen matrix was deteriorated on the DU145 side of the collagen barrier. The DU145-collagen III-hFob model will allow an evaluation of the influence of the matrix on prostate cancer-bone cell interaction and regulation by growth factors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 241-253 |
| Number of pages | 13 |
| Journal | Journal of Submicroscopic Cytology and Pathology |
| Volume | 34 |
| Issue number | 3 |
| State | Published - Jul 2002 |
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Keywords
- DU 145
- HT29
- Type III collagen
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Cell Biology
- Histology
Cite this
Culture and co-culture of DU145 prostate carcinoma, osteoblasts and HT-29 colon carcinoma cells on a fabricated type III collagen matrix. / Macias, C. L.; Williams, K.; Baccetti, B.; Renieri, T.; Reddi, A Hari; Asseffa, A.; Hollis, V.; Grissom, F.; Anderson, W.
In: Journal of Submicroscopic Cytology and Pathology, Vol. 34, No. 3, 07.2002, p. 241-253.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Culture and co-culture of DU145 prostate carcinoma, osteoblasts and HT-29 colon carcinoma cells on a fabricated type III collagen matrix
AU - Macias, C. L.
AU - Williams, K.
AU - Baccetti, B.
AU - Renieri, T.
AU - Reddi, A Hari
AU - Asseffa, A.
AU - Hollis, V.
AU - Grissom, F.
AU - Anderson, W.
PY - 2002/7
Y1 - 2002/7
N2 - DU145 prostate carcinoma cells cultured on type III collagen possessed a highly migratory potential which was twice as much as HT-29 colon carcinoma cells. Prior to attachment to collagen, DU145 cells were highly reactive for fibronectin and after attachment clear zones between cells and collagen suggested protease activity. HT-29 cells attached to type III collagen forming dome-like polyps, however, right and/or gap junctions were not observed. hFob osteoblasts were co-cultured with DU145 to establish a prostate cancer-collagen matrix barrier-bone cell metastasis model. Osteoblasts maintained their differentiated osceoblastic characteristics on one side of the collagen barrier, demonstrating high alkaline phosphatase, osteocalcin and insulin growth factor (IGF) activities. hFob cell growth was prominent adjacent to demineralized bone matrix particles (BMPs) embedded in type III collagen. The collagen matrix was deteriorated on the DU145 side of the collagen barrier. The DU145-collagen III-hFob model will allow an evaluation of the influence of the matrix on prostate cancer-bone cell interaction and regulation by growth factors.
AB - DU145 prostate carcinoma cells cultured on type III collagen possessed a highly migratory potential which was twice as much as HT-29 colon carcinoma cells. Prior to attachment to collagen, DU145 cells were highly reactive for fibronectin and after attachment clear zones between cells and collagen suggested protease activity. HT-29 cells attached to type III collagen forming dome-like polyps, however, right and/or gap junctions were not observed. hFob osteoblasts were co-cultured with DU145 to establish a prostate cancer-collagen matrix barrier-bone cell metastasis model. Osteoblasts maintained their differentiated osceoblastic characteristics on one side of the collagen barrier, demonstrating high alkaline phosphatase, osteocalcin and insulin growth factor (IGF) activities. hFob cell growth was prominent adjacent to demineralized bone matrix particles (BMPs) embedded in type III collagen. The collagen matrix was deteriorated on the DU145 side of the collagen barrier. The DU145-collagen III-hFob model will allow an evaluation of the influence of the matrix on prostate cancer-bone cell interaction and regulation by growth factors.
KW - DU 145
KW - HT29
KW - Type III collagen
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UR - http://www.scopus.com/inward/citedby.url?scp=0036652193&partnerID=8YFLogxK
M3 - Article
VL - 34
SP - 241
EP - 253
JO - Journal of Submicroscopic Cytology and Pathology
JF - Journal of Submicroscopic Cytology and Pathology
SN - 1122-9497
IS - 3
ER -