Abstract
Primary epithelial cell cultures were developed from fetal caruncular, adult intercaruncular pregnant, and adult intercaruncular nonpregnant endometrium. Endometrial cells were obtained from both collagenase dispersion or from explant outgrowth, although the explant method was more consistent. Cultures derived from fetal endometrium grew more rapidly and were more viable after subculturing and cryopreservation than were adult-derived cultures. These cultures provide an in vitro system for the study of bovine endometrial physiology and pathology.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 129-133 |
| Number of pages | 5 |
| Journal | Journal of Tissue Culture Methods |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1988 |
| Externally published | Yes |
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Keywords
- bovine
- endometrium
- fetal
- tissue culture
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Anatomy
Cite this
Cultivation of bovine fetal and adult endometrial epithelial cells. / Munson, Linda; Chandler, Suzanne K.; Schlafer, Donald H.
In: Journal of Tissue Culture Methods, Vol. 11, No. 3, 09.1988, p. 129-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cultivation of bovine fetal and adult endometrial epithelial cells
AU - Munson, Linda
AU - Chandler, Suzanne K.
AU - Schlafer, Donald H.
PY - 1988/9
Y1 - 1988/9
N2 - Primary epithelial cell cultures were developed from fetal caruncular, adult intercaruncular pregnant, and adult intercaruncular nonpregnant endometrium. Endometrial cells were obtained from both collagenase dispersion or from explant outgrowth, although the explant method was more consistent. Cultures derived from fetal endometrium grew more rapidly and were more viable after subculturing and cryopreservation than were adult-derived cultures. These cultures provide an in vitro system for the study of bovine endometrial physiology and pathology.
AB - Primary epithelial cell cultures were developed from fetal caruncular, adult intercaruncular pregnant, and adult intercaruncular nonpregnant endometrium. Endometrial cells were obtained from both collagenase dispersion or from explant outgrowth, although the explant method was more consistent. Cultures derived from fetal endometrium grew more rapidly and were more viable after subculturing and cryopreservation than were adult-derived cultures. These cultures provide an in vitro system for the study of bovine endometrial physiology and pathology.
KW - bovine
KW - endometrium
KW - fetal
KW - tissue culture
UR - http://www.scopus.com/inward/record.url?scp=0024264533&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024264533&partnerID=8YFLogxK
U2 - 10.1007/BF01404265
DO - 10.1007/BF01404265
M3 - Article
AN - SCOPUS:0024264533
VL - 11
SP - 129
EP - 133
JO - Cytotechnology
JF - Cytotechnology
SN - 0920-9069
IS - 3
ER -