Cultivation of bovine fetal and adult endometrial epithelial cells

Linda Munson; Suzanne K. Chandler; Donald H. Schlafer

doi:10.1007/BF01404265

Cultivation of bovine fetal and adult endometrial epithelial cells

Linda Munson, Suzanne K. Chandler, Donald H. Schlafer

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Primary epithelial cell cultures were developed from fetal caruncular, adult intercaruncular pregnant, and adult intercaruncular nonpregnant endometrium. Endometrial cells were obtained from both collagenase dispersion or from explant outgrowth, although the explant method was more consistent. Cultures derived from fetal endometrium grew more rapidly and were more viable after subculturing and cryopreservation than were adult-derived cultures. These cultures provide an in vitro system for the study of bovine endometrial physiology and pathology.

Original language	English (US)
Pages (from-to)	129-133
Number of pages	5
Journal	Journal of Tissue Culture Methods
Volume	11
Issue number	3
DOIs	https://doi.org/10.1007/BF01404265
State	Published - Sep 1988
Externally published	Yes

Keywords

bovine
endometrium
fetal
tissue culture

ASJC Scopus subject areas

Pathology and Forensic Medicine
Anatomy

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10.1007/BF01404265

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abstract = "Primary epithelial cell cultures were developed from fetal caruncular, adult intercaruncular pregnant, and adult intercaruncular nonpregnant endometrium. Endometrial cells were obtained from both collagenase dispersion or from explant outgrowth, although the explant method was more consistent. Cultures derived from fetal endometrium grew more rapidly and were more viable after subculturing and cryopreservation than were adult-derived cultures. These cultures provide an in vitro system for the study of bovine endometrial physiology and pathology.",

keywords = "bovine, endometrium, fetal, tissue culture",

author = "Linda Munson and Chandler, {Suzanne K.} and Schlafer, {Donald H.}",

year = "1988",

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T1 - Cultivation of bovine fetal and adult endometrial epithelial cells

AU - Munson, Linda

AU - Chandler, Suzanne K.

AU - Schlafer, Donald H.

PY - 1988/9

Y1 - 1988/9

N2 - Primary epithelial cell cultures were developed from fetal caruncular, adult intercaruncular pregnant, and adult intercaruncular nonpregnant endometrium. Endometrial cells were obtained from both collagenase dispersion or from explant outgrowth, although the explant method was more consistent. Cultures derived from fetal endometrium grew more rapidly and were more viable after subculturing and cryopreservation than were adult-derived cultures. These cultures provide an in vitro system for the study of bovine endometrial physiology and pathology.

AB - Primary epithelial cell cultures were developed from fetal caruncular, adult intercaruncular pregnant, and adult intercaruncular nonpregnant endometrium. Endometrial cells were obtained from both collagenase dispersion or from explant outgrowth, although the explant method was more consistent. Cultures derived from fetal endometrium grew more rapidly and were more viable after subculturing and cryopreservation than were adult-derived cultures. These cultures provide an in vitro system for the study of bovine endometrial physiology and pathology.

KW - bovine

KW - endometrium

KW - fetal

KW - tissue culture

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ER -

Cultivation of bovine fetal and adult endometrial epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

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