Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan

John W. Thomford, Patricia A Conrad, Sam R. Telford, Dane Mathiesen, Barbara H. Bowman, Andrew Spielman, Mark L. Eberhard, Barbara L. Herwaldt, Robert E. Quick, David H. Persing

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

An intraerythrocytic protozoan (WA1) recently isolated from a patient in Washington State was shown to be morphologically identical to Babesia microti but biologically and genetically distinct. Continuous growth of WA1 was established in stationary erythrocyte cultures. Hybridization of a chemiluminescent Babesia-specific DNA probe to Southern blots of restriction enzyme-digested genomic DNA showed that WA1 could be distinguished from other Babesia species that were antigenically cross-reactive (Babesia gibsoni and babesial parasites from desert bighorn sheep, Ovis canadensis nelsoni) or known to infect humans (B. microti, Babesia divergens, and Babesia equi), or both. A 1436-bp portion of the nuclear small subunit rRNA gene of WA1 was sequenced and analyzed. Genetic distance analysis showed that WA1 is most closely related to the canine pathogen B. gibsoni and lies within a phylogenetic cluster with Theileria species and B. equi. The methodology described will be useful for improved diagnosis and identification of human protozoal pathogens.

Original languageEnglish (US)
Pages (from-to)1050-1056
Number of pages7
JournalJournal of Infectious Diseases
Volume169
Issue number5
StatePublished - May 1994

Fingerprint

Babesia
Bighorn Sheep
Babesia microti
Theileria
Forensic Anthropology
DNA Probes
Southern Blotting
rRNA Genes
Canidae
Parasites
Erythrocytes
DNA
Enzymes
Growth

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Thomford, J. W., Conrad, P. A., Telford, S. R., Mathiesen, D., Bowman, B. H., Spielman, A., ... Persing, D. H. (1994). Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan. Journal of Infectious Diseases, 169(5), 1050-1056.

Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan. / Thomford, John W.; Conrad, Patricia A; Telford, Sam R.; Mathiesen, Dane; Bowman, Barbara H.; Spielman, Andrew; Eberhard, Mark L.; Herwaldt, Barbara L.; Quick, Robert E.; Persing, David H.

In: Journal of Infectious Diseases, Vol. 169, No. 5, 05.1994, p. 1050-1056.

Research output: Contribution to journalArticle

Thomford, JW, Conrad, PA, Telford, SR, Mathiesen, D, Bowman, BH, Spielman, A, Eberhard, ML, Herwaldt, BL, Quick, RE & Persing, DH 1994, 'Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan', Journal of Infectious Diseases, vol. 169, no. 5, pp. 1050-1056.
Thomford JW, Conrad PA, Telford SR, Mathiesen D, Bowman BH, Spielman A et al. Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan. Journal of Infectious Diseases. 1994 May;169(5):1050-1056.
Thomford, John W. ; Conrad, Patricia A ; Telford, Sam R. ; Mathiesen, Dane ; Bowman, Barbara H. ; Spielman, Andrew ; Eberhard, Mark L. ; Herwaldt, Barbara L. ; Quick, Robert E. ; Persing, David H. / Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan. In: Journal of Infectious Diseases. 1994 ; Vol. 169, No. 5. pp. 1050-1056.
@article{fa3fb85c2e8440d5a5824e1333b58fb7,
title = "Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan",
abstract = "An intraerythrocytic protozoan (WA1) recently isolated from a patient in Washington State was shown to be morphologically identical to Babesia microti but biologically and genetically distinct. Continuous growth of WA1 was established in stationary erythrocyte cultures. Hybridization of a chemiluminescent Babesia-specific DNA probe to Southern blots of restriction enzyme-digested genomic DNA showed that WA1 could be distinguished from other Babesia species that were antigenically cross-reactive (Babesia gibsoni and babesial parasites from desert bighorn sheep, Ovis canadensis nelsoni) or known to infect humans (B. microti, Babesia divergens, and Babesia equi), or both. A 1436-bp portion of the nuclear small subunit rRNA gene of WA1 was sequenced and analyzed. Genetic distance analysis showed that WA1 is most closely related to the canine pathogen B. gibsoni and lies within a phylogenetic cluster with Theileria species and B. equi. The methodology described will be useful for improved diagnosis and identification of human protozoal pathogens.",
author = "Thomford, {John W.} and Conrad, {Patricia A} and Telford, {Sam R.} and Dane Mathiesen and Bowman, {Barbara H.} and Andrew Spielman and Eberhard, {Mark L.} and Herwaldt, {Barbara L.} and Quick, {Robert E.} and Persing, {David H.}",
year = "1994",
month = "5",
language = "English (US)",
volume = "169",
pages = "1050--1056",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Cultivation and phylogenetic characterization of a newly recognized human pathogenic protozoan

AU - Thomford, John W.

AU - Conrad, Patricia A

AU - Telford, Sam R.

AU - Mathiesen, Dane

AU - Bowman, Barbara H.

AU - Spielman, Andrew

AU - Eberhard, Mark L.

AU - Herwaldt, Barbara L.

AU - Quick, Robert E.

AU - Persing, David H.

PY - 1994/5

Y1 - 1994/5

N2 - An intraerythrocytic protozoan (WA1) recently isolated from a patient in Washington State was shown to be morphologically identical to Babesia microti but biologically and genetically distinct. Continuous growth of WA1 was established in stationary erythrocyte cultures. Hybridization of a chemiluminescent Babesia-specific DNA probe to Southern blots of restriction enzyme-digested genomic DNA showed that WA1 could be distinguished from other Babesia species that were antigenically cross-reactive (Babesia gibsoni and babesial parasites from desert bighorn sheep, Ovis canadensis nelsoni) or known to infect humans (B. microti, Babesia divergens, and Babesia equi), or both. A 1436-bp portion of the nuclear small subunit rRNA gene of WA1 was sequenced and analyzed. Genetic distance analysis showed that WA1 is most closely related to the canine pathogen B. gibsoni and lies within a phylogenetic cluster with Theileria species and B. equi. The methodology described will be useful for improved diagnosis and identification of human protozoal pathogens.

AB - An intraerythrocytic protozoan (WA1) recently isolated from a patient in Washington State was shown to be morphologically identical to Babesia microti but biologically and genetically distinct. Continuous growth of WA1 was established in stationary erythrocyte cultures. Hybridization of a chemiluminescent Babesia-specific DNA probe to Southern blots of restriction enzyme-digested genomic DNA showed that WA1 could be distinguished from other Babesia species that were antigenically cross-reactive (Babesia gibsoni and babesial parasites from desert bighorn sheep, Ovis canadensis nelsoni) or known to infect humans (B. microti, Babesia divergens, and Babesia equi), or both. A 1436-bp portion of the nuclear small subunit rRNA gene of WA1 was sequenced and analyzed. Genetic distance analysis showed that WA1 is most closely related to the canine pathogen B. gibsoni and lies within a phylogenetic cluster with Theileria species and B. equi. The methodology described will be useful for improved diagnosis and identification of human protozoal pathogens.

UR - http://www.scopus.com/inward/record.url?scp=0028048405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028048405&partnerID=8YFLogxK

M3 - Article

VL - 169

SP - 1050

EP - 1056

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -