Crystallographic Observation of pH-Induced Conformational Changes in the Amyelois transitella Pheromone-Binding Protein AtraPBP1

Eric di Luccio, Yuko Ishida, Walter S. Leal, David K. Wilson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The navel orangeworm, Amyelois transitella is a major agricultural pest causing large losses in a variety of tree crops. Control of this insect pest may be achieved by interfering with olfactory pathways to block detection of female-produced sex pheromones and consequently, disrupt mating. The first component of this pathway is the pheromone-binding protein AtraPBP1, which recognizes the pheromone and presents it to the odorant receptor housed in a sensory neuron of the male antennae. Release of the ligand depends on a pH-induced conformational change associated with the acidity of the membrane surface. To characterize this conformational change and to understand how pheromones bind, we have determined the high resolution crystal structures of AtraPBP1 in complex with two main constituents of the sex pheromone, i.e., (11Z,13Z)-hexadecadienal and (11Z,13Z)-hexadecadienol. Comparison with the structure of the unliganded form demonstrates a large ~90° movement of the C-terminal helix which is observed in other pheromone- or odorant-binding proteins accompanied by an unpredicted 37° displacement of the N-terminal helix. Molecular dynamic trajectories suggest that the conformational change of the α1 helix facilitates the movement of the C-terminal helix.

Original languageEnglish (US)
Article numbere53840
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 13 2013

Fingerprint

Amyelois transitella
Pheromones
pheromones
binding proteins
Carrier Proteins
Observation
Sex Attractants
sex pheromones
Odorant Receptors
Insect Control
Olfactory Pathways
Pest Control
molecular dynamics
sensory neurons
Sensory Receptor Cells
Molecular Dynamics Simulation
crystal structure
Acidity
antennae
insect pests

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Crystallographic Observation of pH-Induced Conformational Changes in the Amyelois transitella Pheromone-Binding Protein AtraPBP1. / di Luccio, Eric; Ishida, Yuko; Leal, Walter S.; Wilson, David K.

In: PLoS One, Vol. 8, No. 2, e53840, 13.02.2013.

Research output: Contribution to journalArticle

di Luccio, Eric ; Ishida, Yuko ; Leal, Walter S. ; Wilson, David K. / Crystallographic Observation of pH-Induced Conformational Changes in the Amyelois transitella Pheromone-Binding Protein AtraPBP1. In: PLoS One. 2013 ; Vol. 8, No. 2.
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