TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA
AU - Crotty, Justin W.
AU - Etzkorn, Christopher
AU - Barbas, Carlos F.
AU - Segal, David
AU - Horton, Nancy C.
PY - 2005
Y1 - 2005
N2 - Crystals of a designed six-finger zinc-finger protein, Aart, bound to a 22-base-pair duplex DNA containing a consensus binding site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as the precipitating agent. The irregularly shaped crystals belong to space group P1, with unit-cell parameters a = 41.95, b = 71.76, c = 74.73 Å, α = 100.87, β = 96.22, γ = 106.33°. There are most likely to be two protein-DNA complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.95 Å at 100 K, with an Rmerge of 9.3%.
AB - Crystals of a designed six-finger zinc-finger protein, Aart, bound to a 22-base-pair duplex DNA containing a consensus binding site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as the precipitating agent. The irregularly shaped crystals belong to space group P1, with unit-cell parameters a = 41.95, b = 71.76, c = 74.73 Å, α = 100.87, β = 96.22, γ = 106.33°. There are most likely to be two protein-DNA complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.95 Å at 100 K, with an Rmerge of 9.3%.
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U2 - 10.1107/S1744309105014363
DO - 10.1107/S1744309105014363
M3 - Article
C2 - 16511099
AN - SCOPUS:33744483392
VL - 61
SP - 573
EP - 576
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 6
ER -