Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA

Justin W. Crotty; Christopher Etzkorn; Carlos F. Barbas; David Segal; Nancy C. Horton

doi:10.1107/S1744309105014363

Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA

Justin W. Crotty, Christopher Etzkorn, Carlos F. Barbas, David Segal, Nancy C. Horton

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Crystals of a designed six-finger zinc-finger protein, Aart, bound to a 22-base-pair duplex DNA containing a consensus binding site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as the precipitating agent. The irregularly shaped crystals belong to space group P1, with unit-cell parameters a = 41.95, b = 71.76, c = 74.73 Å, α = 100.87, β = 96.22, γ = 106.33°. There are most likely to be two protein-DNA complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.95 Å at 100 K, with an R_merge of 9.3%.

Original language	English (US)
Pages (from-to)	573-576
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	61
Issue number	6
DOIs	https://doi.org/10.1107/S1744309105014363
State	Published - 2005
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Biophysics
Structural Biology
Genetics
Condensed Matter Physics

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Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA. / Crotty, Justin W.; Etzkorn, Christopher; Barbas, Carlos F.; Segal, David; Horton, Nancy C.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 61, No. 6, 2005, p. 573-576.

Research output: Contribution to journal › Article › peer-review

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abstract = "Crystals of a designed six-finger zinc-finger protein, Aart, bound to a 22-base-pair duplex DNA containing a consensus binding site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as the precipitating agent. The irregularly shaped crystals belong to space group P1, with unit-cell parameters a = 41.95, b = 71.76, c = 74.73 {\AA}, α = 100.87, β = 96.22, γ = 106.33°. There are most likely to be two protein-DNA complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.95 {\AA} at 100 K, with an Rmerge of 9.3%.",

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AU - Horton, Nancy C.

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AB - Crystals of a designed six-finger zinc-finger protein, Aart, bound to a 22-base-pair duplex DNA containing a consensus binding site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as the precipitating agent. The irregularly shaped crystals belong to space group P1, with unit-cell parameters a = 41.95, b = 71.76, c = 74.73 Å, α = 100.87, β = 96.22, γ = 106.33°. There are most likely to be two protein-DNA complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.95 Å at 100 K, with an Rmerge of 9.3%.

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Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA

Abstract

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