Crystal structures of unbound and aminooxyacetate-bound Escherichia coli γ-aminobutyrate aminotransferase

Wenshe Liu, Peter E. Peterson, Richard J. Carter, Xianzhi Zhou, James A. Langston, Andrew J Fisher, Michael D. Toney

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Abstract

The X-ray crystal structures of Escherichia coli γ-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3 221 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 Å resolution for the unliganded enzyme and 1.9 Å resolution for the aminooxyacetate complex. The overall structure of the enzyme is similar to those of other aminotransferase subgroup II enzymes. The ability of γ-aminobutyrate aminotransferase to act on primary amine substrates (γ-aminobutyrate) in the first half-reaction and α-amino acids in the second is proposed to be enabled by the presence of Glu211, whose side chain carboxylate alternates between interactions with Arg398 in the primary amine half-reaction and an alternative binding site in the α-amino acid half-reaction, in which Arg398 binds the substrate α-carboxylate. The specificity for a carboxylate group on the substrate side chain is due primarily to the presence of Arg141, but also requires substantial local main chain rearrangements relative to the structurally homologous enzyme dialkylglycine decarboxylase, which is specific for small alkyl side chains. No iron-sulfur cluster is found in the bacterial enzyme as was found in the pig enzyme [Storici, P., De Biase, D., Bossa, F., Bruno, S., Mozzarelli, A., Peneff, C., Silverman, R. B., and Schirmer, T. (2004) J. Biol. Chem. 279, 363-73.]. The binding of aminooxyacetate causes remarkably small changes in the active site structure, and no large domain movements are observed. Active site structure comparisons with pig γ-aminobutyrate aminotransferase and dialkylglycine decarboxylase are discussed.

Original languageEnglish (US)
Pages (from-to)10896-10905
Number of pages10
JournalBiochemistry
Volume43
Issue number34
DOIs
StatePublished - Aug 31 2004

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Liu, W., Peterson, P. E., Carter, R. J., Zhou, X., Langston, J. A., Fisher, A. J., & Toney, M. D. (2004). Crystal structures of unbound and aminooxyacetate-bound Escherichia coli γ-aminobutyrate aminotransferase. Biochemistry, 43(34), 10896-10905. https://doi.org/10.1021/bi049218e