Crystal structures of sialyltransferase from Photobacterium damselae

Nhung Huynh, Yanhong Li, Hai Yu, Shengshu Huang, Kam Lau, Xi Chen, Andrew J Fisher

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Sialyltransferase structures fall into either GT-A or GT-B glycosyltransferase fold. Some sialyltransferases from the Photobacterium genus have been shown to contain an additional N-terminal immunoglobulin (Ig)-like domain. Photobacterium damselae α2-6-sialyltransferase has been used efficiently in enzymatic and chemoenzymatic synthesis of α2-6-linked sialosides. Here we report three crystal structures of this enzyme. Two structures with and without a donor substrate analog CMP-3F(a)Neu5Ac contain an immunoglobulin (Ig)-like domain and adopt the GT-B sialyltransferase fold. The binary structure reveals a non-productive pre-Michaelis complex, which are caused by crystal lattice contacts that prevent the large conformational changes. The third structure lacks the Ig-domain. Comparison of the three structures reveals small inherent flexibility between the two Rossmann-like domains of the GT-B fold.

Original languageEnglish (US)
Pages (from-to)4720-4729
Number of pages10
JournalFEBS Letters
Volume588
Issue number24
DOIs
StatePublished - Dec 20 2014

Fingerprint

Photobacterium
Sialyltransferases
Crystal structure
Immunoglobulins
Cytidine Monophosphate
Glycosyltransferases
Crystal lattices
Substrates
Enzymes
Immunoglobulin Domains

Keywords

  • CMP-3F(a)Neu5Ac
  • Photobacterium damselae
  • Protein crystal structure
  • Sialic acid
  • α 2-6-Sialyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Crystal structures of sialyltransferase from Photobacterium damselae. / Huynh, Nhung; Li, Yanhong; Yu, Hai; Huang, Shengshu; Lau, Kam; Chen, Xi; Fisher, Andrew J.

In: FEBS Letters, Vol. 588, No. 24, 20.12.2014, p. 4720-4729.

Research output: Contribution to journalArticle

Huynh, Nhung ; Li, Yanhong ; Yu, Hai ; Huang, Shengshu ; Lau, Kam ; Chen, Xi ; Fisher, Andrew J. / Crystal structures of sialyltransferase from Photobacterium damselae. In: FEBS Letters. 2014 ; Vol. 588, No. 24. pp. 4720-4729.
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AU - Fisher, Andrew J

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