Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks

Martin R. Singleton, Mark S. Dillingham, Martin Gaudier, Stephen C. Kowalczykowski, Dale B. Wigley

Research output: Contribution to journalArticle

279 Citations (Scopus)

Abstract

RecBCD is a multi-functional enzyme complex that processes DNA ends resulting from a double-strand break. RecBCD is a bipolar helicase that splits the duplex into its component strands and digests them until encountering a recombinational hotspot (Chi site). The nuclease activity is then attenuated and RecBCD loads RecA onto the 3′ tail of the DNA. Here we present the crystal structure of RecBCD bound to a DNA substrate. In this initiation complex, the DNA duplex has been split across the RecC subunit to create a fork with the separated strands each heading towards different helicase motor subunits. The strands pass along tunnels within the complex, both emerging adjacent to the nuclease domain of RecB. Passage of the 3′ tail through one of these tunnels provides a mechanism for the recognition of a Chi sequence by RecC within the context of double-stranded DNA. Gating of this tunnel suggests how nuclease activity might be regulated.

Original languageEnglish (US)
Pages (from-to)187-193
Number of pages7
JournalNature
Volume432
Issue number7014
DOIs
StatePublished - Nov 11 2004

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Exodeoxyribonuclease V
DNA Breaks
DNA
Enzymes

ASJC Scopus subject areas

  • General

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Singleton, M. R., Dillingham, M. S., Gaudier, M., Kowalczykowski, S. C., & Wigley, D. B. (2004). Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature, 432(7014), 187-193. https://doi.org/10.1038/nature02988

Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. / Singleton, Martin R.; Dillingham, Mark S.; Gaudier, Martin; Kowalczykowski, Stephen C.; Wigley, Dale B.

In: Nature, Vol. 432, No. 7014, 11.11.2004, p. 187-193.

Research output: Contribution to journalArticle

Singleton, MR, Dillingham, MS, Gaudier, M, Kowalczykowski, SC & Wigley, DB 2004, 'Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks', Nature, vol. 432, no. 7014, pp. 187-193. https://doi.org/10.1038/nature02988
Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB. Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. Nature. 2004 Nov 11;432(7014):187-193. https://doi.org/10.1038/nature02988
Singleton, Martin R. ; Dillingham, Mark S. ; Gaudier, Martin ; Kowalczykowski, Stephen C. ; Wigley, Dale B. / Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. In: Nature. 2004 ; Vol. 432, No. 7014. pp. 187-193.
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