Crystal Structure of Baculovirus P35 Reveals a Novel Conformational Change in the Reactive Site Loop after Caspase Cleavage

Wilfred P. Dela Cruz, Paul D. Friesen, Andrew J Fisher

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Baculovirus P35 is a universal suppressor of apoptosis that stoichiometrically inhibits cellular caspases in a novel cleavage-dependent mechanism. Upon caspase cleavage at Asp-87, the 10- and 25-kDa cleavage products of P35 remain tightly associated with the inhibited caspase. Mutations in the α-helix of the reactive site loop preceding the cleavage site abrogate caspase inhibition and antiapoptotic activity. Substitution of Pro for Val-71, which is located in the middle of this α-helix, produces a protein that is cleaved at the requisite Asp-87 but does not remain bound to the caspase. This loss-of-function mutation provided the opportunity to structurally analyze the conformational changes of the P35 reactive site loop after caspase cleavage. We report here the 2.7 Å resolution crystal structure of V71P-mutated P35 after cleavage by human caspase-3. The structure reveals a large movement in the carboxyl-terminal side of the reactive site loop that swings down and forms a new β-strand that augments an existing β-sheet. Additionally, the hydrophobic amino terminus releases and extends away from the protein core. Similar movements occur when P35 forms an inhibitory complex with human caspase-8. These findings suggest that the α-helix mutation may alter the sequential steps or kinetics of the conformational changes required for inhibition, thereby causing P35 loss of function.

Original languageEnglish (US)
Pages (from-to)32933-32939
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number35
DOIs
StatePublished - Aug 31 2001

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Baculoviridae
Caspases
Catalytic Domain
Crystal structure
Mutation
Caspase 3
Proteins
Substitution reactions
Apoptosis
Kinetics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Crystal Structure of Baculovirus P35 Reveals a Novel Conformational Change in the Reactive Site Loop after Caspase Cleavage. / Dela Cruz, Wilfred P.; Friesen, Paul D.; Fisher, Andrew J.

In: Journal of Biological Chemistry, Vol. 276, No. 35, 31.08.2001, p. 32933-32939.

Research output: Contribution to journalArticle

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