Crystal Structure of an Invertebrate Caspase

Charles M. Forsyth, Donna Lemongello, Douglas J. LaCount, Paul D. Friesen, Andrew J Fisher

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Caspases play an essential role in the execution of apoptosis. These cysteine proteases are highly conserved among metazoans and are translated as inactive zymogens, which are activated by proteolytic cleavages to generate the large and small subunits and remove the N-terminal prodomain. The 2.3 Å resolution crystal structure of active Sf-caspase-1, the principal effector caspase of the insect Spodoptera frugiperda, is presented here. The structure represents the first nonhuman caspase to be resolved. The structure of the cleaved and active protease was determined with the tetrapeptide inhibitor N-acetyl-Asp-Glu-Val-Asp-chloromethylketone covalently bonded to the active site cysteine. As expected, the overall fold of Sf-caspase-1 is exceedingly similar to that of the five active caspases from humans solved to date. The overall structure and active site arrangement of Sf-caspase-1 is most comparable with that of the human effector caspases, with which it shares highest sequence homology. The most prominent structural difference with Sf-caspase-1 is the position of the N-terminal region of the large subunit. Unlike the N terminus of human caspases, the N terminus of Sf-caspase-1 originates from the active site side where it interacts with active site loop L2 and then extends to the backside of the heterodimer. This unusual structural arrangement raises the possibility that the N-terminal prodomain plays a regulatory role during effector caspase activation or enzyme activity in insects.

Original languageEnglish (US)
Pages (from-to)7001-7008
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number8
DOIs
StatePublished - Feb 20 2004

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Caspase 1
Invertebrates
Caspases
Effector Caspases
Crystal structure
Catalytic Domain
Insects
Spodoptera
Enzyme Precursors
Enzyme Activation
Cysteine Proteases
Enzyme activity
Sequence Homology
Cysteine
Peptide Hydrolases
Chemical activation
Apoptosis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Forsyth, C. M., Lemongello, D., LaCount, D. J., Friesen, P. D., & Fisher, A. J. (2004). Crystal Structure of an Invertebrate Caspase. Journal of Biological Chemistry, 279(8), 7001-7008. https://doi.org/10.1074/jbc.M312472200

Crystal Structure of an Invertebrate Caspase. / Forsyth, Charles M.; Lemongello, Donna; LaCount, Douglas J.; Friesen, Paul D.; Fisher, Andrew J.

In: Journal of Biological Chemistry, Vol. 279, No. 8, 20.02.2004, p. 7001-7008.

Research output: Contribution to journalArticle

Forsyth, CM, Lemongello, D, LaCount, DJ, Friesen, PD & Fisher, AJ 2004, 'Crystal Structure of an Invertebrate Caspase', Journal of Biological Chemistry, vol. 279, no. 8, pp. 7001-7008. https://doi.org/10.1074/jbc.M312472200
Forsyth CM, Lemongello D, LaCount DJ, Friesen PD, Fisher AJ. Crystal Structure of an Invertebrate Caspase. Journal of Biological Chemistry. 2004 Feb 20;279(8):7001-7008. https://doi.org/10.1074/jbc.M312472200
Forsyth, Charles M. ; Lemongello, Donna ; LaCount, Douglas J. ; Friesen, Paul D. ; Fisher, Andrew J. / Crystal Structure of an Invertebrate Caspase. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 8. pp. 7001-7008.
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