Cryptotomography: Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns

N. D. Loh; M. J. Bogan; V. Elser; A. Barty; S. Boutet; S. Bajt; J. Hajdu; T. Ekeberg; F. R.N.C. Maia; J. Schulz; M. M. Seibert; B. Iwan; N. Timneanu; S. Marchesini; I. Schlichting; R. L. Shoeman; L. Lomb; Matthias Frank; M. Liang; H. N. Chapman

doi:10.1103/PhysRevLett.104.225501

Cryptotomography: Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns

N. D. Loh, M. J. Bogan, V. Elser, A. Barty, S. Boutet, S. Bajt, J. Hajdu, T. Ekeberg, F. R.N.C. Maia, J. Schulz, M. M. Seibert, B. Iwan, N. Timneanu, S. Marchesini, I. Schlichting, R. L. Shoeman, L. Lomb, Matthias Frank, M. Liang, H. N. Chapman

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.

Original language	English (US)
Article number	225501
Journal	Physical Review Letters
Volume	104
Issue number	22
DOIs	https://doi.org/10.1103/PhysRevLett.104.225501
State	Published - Jun 2 2010
Externally published	Yes

ASJC Scopus subject areas

Physics and Astronomy(all)

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Loh, N. D., Bogan, M. J., Elser, V., Barty, A., Boutet, S., Bajt, S., Hajdu, J., Ekeberg, T., Maia, F. R. N. C., Schulz, J., Seibert, M. M., Iwan, B., Timneanu, N., Marchesini, S., Schlichting, I., Shoeman, R. L., Lomb, L., Frank, M., Liang, M., & Chapman, H. N. (2010). Cryptotomography: Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns. Physical Review Letters, 104(22), [225501]. https://doi.org/10.1103/PhysRevLett.104.225501

Cryptotomography : Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns. / Loh, N. D.; Bogan, M. J.; Elser, V.; Barty, A.; Boutet, S.; Bajt, S.; Hajdu, J.; Ekeberg, T.; Maia, F. R.N.C.; Schulz, J.; Seibert, M. M.; Iwan, B.; Timneanu, N.; Marchesini, S.; Schlichting, I.; Shoeman, R. L.; Lomb, L.; Frank, Matthias; Liang, M.; Chapman, H. N.

In: Physical Review Letters, Vol. 104, No. 22, 225501, 02.06.2010.

Research output: Contribution to journal › Article › peer-review

Loh, ND, Bogan, MJ, Elser, V, Barty, A, Boutet, S, Bajt, S, Hajdu, J, Ekeberg, T, Maia, FRNC, Schulz, J, Seibert, MM, Iwan, B, Timneanu, N, Marchesini, S, Schlichting, I, Shoeman, RL, Lomb, L, Frank, M, Liang, M & Chapman, HN 2010, 'Cryptotomography: Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns', Physical Review Letters, vol. 104, no. 22, 225501. https://doi.org/10.1103/PhysRevLett.104.225501

Loh, N. D. ; Bogan, M. J. ; Elser, V. ; Barty, A. ; Boutet, S. ; Bajt, S. ; Hajdu, J. ; Ekeberg, T. ; Maia, F. R.N.C. ; Schulz, J. ; Seibert, M. M. ; Iwan, B. ; Timneanu, N. ; Marchesini, S. ; Schlichting, I. ; Shoeman, R. L. ; Lomb, L. ; Frank, Matthias ; Liang, M. ; Chapman, H. N. / Cryptotomography : Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns. In: Physical Review Letters. 2010 ; Vol. 104, No. 22.

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AU - Barty, A.

AU - Boutet, S.

AU - Bajt, S.

AU - Hajdu, J.

AU - Ekeberg, T.

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AU - Seibert, M. M.

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AU - Timneanu, N.

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AU - Schlichting, I.

AU - Shoeman, R. L.

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AU - Frank, Matthias

AU - Liang, M.

AU - Chapman, H. N.

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Cryptotomography: Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns

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