Cryptotomography: Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns

N. D. Loh, M. J. Bogan, V. Elser, A. Barty, S. Boutet, S. Bajt, J. Hajdu, T. Ekeberg, F. R.N.C. Maia, J. Schulz, M. M. Seibert, B. Iwan, N. Timneanu, S. Marchesini, I. Schlichting, R. L. Shoeman, L. Lomb, Matthias Frank, M. Liang, H. N. Chapman

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77 Scopus citations

Abstract

We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.

Original languageEnglish (US)
Article number225501
JournalPhysical Review Letters
Volume104
Issue number22
DOIs
StatePublished - Jun 2 2010
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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    Loh, N. D., Bogan, M. J., Elser, V., Barty, A., Boutet, S., Bajt, S., Hajdu, J., Ekeberg, T., Maia, F. R. N. C., Schulz, J., Seibert, M. M., Iwan, B., Timneanu, N., Marchesini, S., Schlichting, I., Shoeman, R. L., Lomb, L., Frank, M., Liang, M., & Chapman, H. N. (2010). Cryptotomography: Reconstructing 3D fourier intensities from randomly oriented single-shot diffraction patterns. Physical Review Letters, 104(22), [225501]. https://doi.org/10.1103/PhysRevLett.104.225501