Abstract
We reconstructed the 3D Fourier intensity distribution of monodisperse prolate nanoparticles using single-shot 2D coherent diffraction patterns collected at DESY's FLASH facility when a bright, coherent, ultrafast x-ray pulse intercepted individual particles of random, unmeasured orientations. This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter. This work is an important step towards realizing single-shot diffraction imaging of single biomolecules.
Original language | English (US) |
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Article number | 225501 |
Journal | Physical Review Letters |
Volume | 104 |
Issue number | 22 |
DOIs | |
State | Published - Jun 2 2010 |
Externally published | Yes |
ASJC Scopus subject areas
- Physics and Astronomy(all)