Cryptosporidium and Giardia in marine-foraging river otters (Lontra canadensis) from the Puget Sound Georgia Basin ecosystem

J. K. Gaydos, Woutrina A Smith, Kirsten Vk Gilardi, A. Melli, H. Schwantje, C. Engelstoft, Heather Fritz, Patricia A Conrad

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Species of Cryptosporidium and Giardia can infect humans and wildlife and have the potential to be transmitted between these 2 groups; yet, very little is known about these protozoans in marine wildlife. Feces of river otters (Lontra canadensis), a common marine wildlife species in the Puget Sound Georgia Basin, were examined for species of Cryptosporidium and Giardia to determine their role in the epidemiology of these pathogens. Using ZnSO4 flotation and immunomagnetic separation, followed by direct immunofluorescent antibody detection (IMS/DFA), we identified Cryptosporidium sp. oocysts in 9 fecal samples from 6 locations and Giardia sp. cysts in 11 fecal samples from 7 locations. The putative risk factors of proximate human population and degree of anthropogenic shoreline modification were not associated with the detection of Cryptosporidium or Giardia spp. in river otter feces. Amplification of DNA from the IMS/DFA slide scrapings was successful for 1 sample containing >500 Cryptosporidium sp. oocysts. Sequences from the Cryptosporidium 18S rRNA and the COWP loci were most similar to the ferret Cryptosporidium sp. genotype. River otters could serve as reservoirs for Cryptosporidium and Giardia species in marine ecosystems. More work is needed to better understand the zoonotic potential of the genotypes they carry as well as their implications for river otter health.

Original languageEnglish (US)
Pages (from-to)198-202
Number of pages5
JournalJournal of Parasitology
Volume93
Issue number1
DOIs
StatePublished - Feb 2007

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Otters
Lontra canadensis
Giardia
Cryptosporidium
Rivers
Ecosystem
foraging
basins
ecosystems
ecosystem
basin
river
feces
genotype
wildlife
Oocysts
epidemiology
cyst
risk factor
oocysts

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Cryptosporidium and Giardia in marine-foraging river otters (Lontra canadensis) from the Puget Sound Georgia Basin ecosystem. / Gaydos, J. K.; Smith, Woutrina A; Gilardi, Kirsten Vk; Melli, A.; Schwantje, H.; Engelstoft, C.; Fritz, Heather; Conrad, Patricia A.

In: Journal of Parasitology, Vol. 93, No. 1, 02.2007, p. 198-202.

Research output: Contribution to journalArticle

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